地黄对百草枯解毒机理的研究  

Detoxication mechanism of Rehmannia glutinosa to paraquat

作　　者：徐凤[1] 杨德亮[1] 杨杰[1] 刘秀[1] 张闯[1] 吴明根[1] 

机构地区：[1]延边大学农学院,延吉133000

出　　处：《植物保护》2014年第1期134-136,共3页Plant Protection

基　　金：国家自然科学基金(31160370)

摘　　要：地黄对百草枯表现出较强的耐药性,百草枯分别处理300g/hm2或600g/hm2时,药害等级分别为0级和2级,且药害症状恢复较快。研究结果表明,地黄块根、叶片乙醇提取物的DPPH自由基清除率随着提取物浓度的增加而增高;叶片对DPPH自由基的清除效果好于块根,DPPH自由基清除率为50%时所需的地黄块根、叶片乙醇提取物浓度分别为20.0g/L和12.2g/L。可以推测,地黄对百草枯的解毒是因为体内含有抗氧化活性物质,这种物质遇到百草枯时能够解除百草枯活性氧毒性,起到地黄对百草枯的解毒作用。Rehmannia glutinosa showed tolerance to paraquat. The phytotoxicity of R. glutinosa was grade 0 and grade 2 with paraquat at 300 g/hm2 or 600 g/hm2, respectively. The results showed that the DPPH free radical scavenging activity of ethanol extract from R. glutinosa root tubers and leaves increased with the extractive con- centration adding; the DPPH scavenging effect in the leaves was better than that in the root tubers. The concen- trations of R. glutinosa root tubers and leaves extracted with ethanol were 20.0 g/L and 12.2 g/L, respectively, when the DPPH free radical scavenging activity was 50%. In general, the detoxication of R. glutinosa to paraquat was caused by the antioxidant substance which could remove paraquat reactive oxygen toxicity.

关 键 词：地黄 百草枯 抗氧化 

分 类 号：S481.4[农业科学—农药学]