神秘果叶挥发油化学成分分析及抗菌、抗肿瘤活性(英文)  

Chemical Composition of Leaf Essential Oil of Synsepalum dulcificum and Evaluation of Its Antibacterial and Antitumoral Activities in vitro

作　　者：卢圣楼[1] 刘红[1] 陈光英[1] 韩长日[1] 臧文霞[1] 

机构地区：[1]海南师范大学化学与化工学院,热带药用植物化学教育部重点实验室,海南海口571127

出　　处：《林产化学与工业》2014年第1期121-127,共7页Chemistry and Industry of Forest Products

基　　金：科技部973前期研究专项课题(2011CB512010);海南省高等学校科学研究基金项目(Hjkj2012-22);海南省高等学校优秀中青年骨干教师基金项目(无编号)

摘　　要：采用水蒸气蒸馏法提取海南产神秘果叶挥发油，利用气相色谱－质谱联用技术（GC-MS）分析挥发油的化学成分，并对挥发油的抑菌和抗肿瘤活性进行了评价。结果从挥发油中共分离出68个化合物，鉴定出44种化学成分，占挥发油总质量分数的92．14％。其中质量分数较高的组分为匙叶桉油烯醇（24．194％），柠檬烯（15．805％），邻苯二甲酸二异辛酯（12．402％），邻苯二甲酸二丁酯（10．326％），棕榈酸（4．865％）和芳樟醇（2．139％）。体外抗菌试验的结果表明，神秘果叶挥发油除了对绿脓杆菌没有明显的抑制作用，对其他7个试验菌株均具有较好的抑菌活性，最小抑茵浓度（MIC）范围为39．06～252．15mg／L。抗肿瘤活性试验的结果表明，神秘果叶挥发油能够有效抑制人白血病细胞K562的增殖，丰数抑制浓度（IC50）值为13．5mg／L。The aim of this study was to determine the chemical composition of Synsepalum dulcificum leaf essential oil obtained by steam distillation. The in vitro antibacterial activity and antitumoral activity of this essential oil was characterized. Sixty-eight compositions were separated. Among them 44 components were identified and represented 92.14% of the total detected constituents. The major chemical compounds of leaf essential oil were spathulenol （24. 194% ）, limonene （ 15. 805% ）, diisooctyl phthalate （ 12. 402% ）, dibutyl phthalate （ 10. 326% ）, palmitic acid （4. 865% ） and linalool （2. 139% ）. The result of the antimicrobial assay showed that the essential oil displayed varying degrees of antibacterial activity against all tested bacterial except for Pseudomonas aeruginosa, along with minimum inhibition concentration（MIC） values from 39.06 to 252. 15 mg/L. In addition, the antitumoral activity using MTY assay of the leaf essential oil exhibited this oil was effective against human K562 cancer cell line in a dose dependent manner. Its inhibition concentration 50% （ IC50 ） value was 13.5 mg/L.

关 键 词：神秘果叶 挥发油 化学成分 抗菌活性 抗肿瘤活性 

分 类 号：TQ351[化学工程]