国产血竭总黄酮成分的纯化及活性成分的含量测定  

Purification of total flavones by macroporous resin and its determination of the active components from Resina Draconis

作　　者：张明媛[1,2] 宓鹤鸣[1] 范国荣[1] 陆峰[1] 亓云鹏[1] 

机构地区：[1]第二军医大学药学院药物分析学教研室,上海200433 [2]福建医科大学附属南平第一医院药学部,福建南平353000

出　　处：《药学实践杂志》2014年第1期42-44,共3页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(30901981);第二军医大学药学院"时珍学者培养计划"资助

摘　　要：目的 采用大孔树脂对国产血竭总黄酮进行分离纯化,并测定其中活性成分的含量.方法 收集大孔树脂70％乙醇和95％乙醇洗脱液,采用高效液相色谱（HPLC）法测定其中活性成分——龙血素A、龙血素B的含量.以ODS柱为分析柱,乙腈：1％冰醋酸（34.5∶65.5）为流动相,检测波长为280 nm.结果 龙血素A、龙血素B分别在11.00 ～ 275.00 g/ml、20.00～500.00 g/ml范围内线性关系良好,线性方程分别为Y=35 844C ＋44 725（r =0.999 9）,Y=28 533C-41 085（r =0.999 9）；方法的准确度、精密度、稳定性均符合要求.制得的国产血竭精制总黄酮中龙血素A、龙血素B的含量分别为26.93、25.53 mg/g.结论 本法可用于国产血竭中相关活性成分的分析及制备,为进一步研究国产血竭活血化瘀作用提供依据.Objective To purify the total flavones from Resina Draconis using D10I resin, and set up a method of simultaneous- ly determining the contents of loureirin A and B in the extract. Methods Fractions in 70% and 95% ethanol were collected. Mixture of acetonitrile and 1% acetic acid （34.5 ： 65.5） was used as the mobile phase and ODS column was used as stationary phase to determine loureirin A and B. The detecting wave length was 280 nm. Results In the established HPLC method, the linear range of loureirin A was 11.00 - 275. O0 g/ml, and that of loureirin B was 20.00 - 500.00 g/ml. Linear equation of loureirin A was Y = 35 844C ＋ 44 725 （ r = 0. 999 9 ） and that of loureirin B was Y = 28 533 C - 41 085, r = 0. 999 9. The accuracy, precision and stability of this method were satisfactory. Conclusion The proposed method was suitable for preparation of total flavones and determination of its active compo- nents front Resina Draconis.

关 键 词：国产血竭总黄酮 大孔树脂 

分 类 号：R917[医药卫生—药物分析学] R28[医药卫生—药学]