抗肿瘤多肽蜂毒素的固相合成  

Solid-phase synthesis of melittin of anti-tumor peptide

作　　者：李文娟[1] 李任武[2] 吴茂诚[2] 王傅喆[3] 秦丽萍[3] 李柏[3] 胡宏岗[2] 

机构地区：[1]烟台大学药学院,山东烟台264005 [2]第二军医大学药学院有机化学教研室,上海200433 [3]第二军医大学长海医院中医系,上海200433

出　　处：《药学实践杂志》2014年第1期51-52,共2页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(20902109)

摘　　要：目的合成具有抗肿瘤活性的二十六肽蜂毒素。方法采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/DCC为缩合剂的合成工艺。结果通过本法能够顺利合成得到二十六肽蜂毒素,收率达32%。结论该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成。Objective To synthesize the antitumor activity of twenty-six peptide melittin. Methods The melittin was synthe- sized by stepwise solid-phase peptide synthesis （ SPPS ）. During the synthesis of melittin, wang-resin was chosen as solid supporter and HOBt/ DCC were used as the coupling reagent. Results The melittin was synthesized with yield of 32%. Conclusion The syn- thetic method was feasible, simple and with high yield for long chain polypeptide synthesis.

关 键 词：蜂毒素 固相合成 逐步缩合 

分 类 号：R996.3[医药卫生—毒理学] R914.5[医药卫生—药学]