HPLC法测定双黄消炎片中黄芩苷的含量  

Determination of Baicalin in Shuanghuangxiaoyan Tablets by HPLC

作　　者：王国凯[1] 刘劲松[1] 

机构地区：[1]安徽中医药大学药学院,现代中药安徽省重点实验室,安徽合肥230031

出　　处：《广州化工》2014年第4期105-107,共3页GuangZhou Chemical Industry

摘　　要：建立高效液相法测定双黄消炎片中黄芩苷含量的方法。色谱柱为汉邦（4．60mm×250mm，5μm），流动相为甲醇-0．1％磷酸=（50：50），流速1．0mL·min^-1；检测波长为274mm。黄芩苷在17．6～176．0μg·mL^-1浓度范围与峰面积呈良好的线性关系（R=0．9991），平均回收率为98．14％，RSD=0．99％。本方法简便、准确、专属性强、重现性好，可用于双黄消炎片中黄芩苷的含量测定。The determination method of baicalin in Shuanghuangxiaoyan Tablets was established by HPLC. The hanbon CIS （4.60mm×250mm,5μm） column was used. The mobile phases consisted of methanol - 0. 1 % phosphoric acid, the flow rate was 1.0mL·min^-1 and the detection wavelength was 274 nm. The linear concentration range of scutellarin was at 17.6～176.0μg·mL^-1 （R = 0.9991）. The average recovery was 98. 14%, and RSD was 0. 99%. The method was simple, reliable, and accurate and can be used in the quality control of baicalin in Shuanghuangxiaoyan Tablets.

关 键 词：双黄消炎片 黄芩苷 HPLC 含量测定 

分 类 号：R285.5[医药卫生—中药学]