糖尿病大鼠胃黏膜细胞色素C异常表达及大黄素调控  

Effect of emodin on expression of cytochrome c in gastric mucosa of diabetic rats

作　　者：徐富翠[1] 赵宏贤[1] 杨晓红[1] 郭勇[1] 

机构地区：[1]泸州医学院基础医学院组胚教研室,四川泸州646000

出　　处：《泸州医学院学报》2014年第1期83-86,共4页Journal of Luzhou Medical College

基　　金：四川省卫生厅资助项目(100242);泸州市科技局重点项目(2009-S-15(5/7)

摘　　要：目的:探讨糖尿病胃病的相关机制及大黄素干预。方法:SD大鼠随机分为对照组(正常组)、实验组(糖尿病组)与大黄素组,实验组应用腹腔一次性注射链脲佐菌素(streptozotocin,STZ)建立糖尿病大鼠模型,造模成功10周后免疫组织化学检测胃黏膜腺细胞细胞色素C(cytc)的表达情况。结果:①实验组和大黄素组大鼠于造模后均出现多尿、多饮、多食症状,体重减轻,平均体重明显低下对照组(P<0.05);平均血糖浓度显著高于对照组(P<0.05)等糖尿病症状。但应用大黄素干预组大鼠的血糖浓度显著低于实验组。②实验组cyt c蛋白阳性表达的胃黏膜细胞数明显高于对照组(P<0.05;大黄素组cyt c蛋白阳性表达的胃黏膜细胞数明显少于实验组(P<0.05)。结论:糖尿病引起大鼠胃黏膜细胞高表达cyt c蛋白,导致细胞死亡增加,参与糖尿病胃病的发生,而大黄素可降低胃黏膜细胞cyt c蛋白表达,从而缓解糖尿病胃病。Objective：To investigate the pathegenetic mechanism and effect of emodin on gastric disturbance of diabetic rats. Methods：SD rats were randomly divided into control group, diabetic group and emodin group. Ten weeks later, expression of cytochrome C in gastric mucosa was detected by immunohistochemistry. Results： ①In diabetic group and emodin group, typical symptoms of diabetes appeared, the average weight was significantly lower （P〈0.05）, and the blood glucose was significantly higher than those in control group （P〈0.05）.②Compared with rats in control group, the cytochrome C in diabetic group was significantly higher （P〈0.05）. Compared with diabetic group, the cytochrome C in emodin group was significantly lower （P〈0.05）. Conclusion：Diabetes causes high expression of cytochrome C in gastric mucosal cells, which induces cell death and gastric disturbance of diabetic rats. Emodin may decrease cytochrome C in gastric mucosa and therefore,alleviate the gastric disturbance of betic rats.

关 键 词：糖尿病 胃黏膜 细胞色素C 细胞凋亡 大黄素 

分 类 号：R587.1[医药卫生—内分泌] Q244[医药卫生—内科学]