潜阳合剂对自发性高血压大鼠胸主动脉重构的影响  

Effects of Qianyang Heji Decoction on the Arterial Remodeling of the Thoracic Aorta in Spontaneously Hypertensive Rats

作　　者：刘春[1] 蒋梅先[2] 

机构地区：[1]河南中医学院第三附属医院 [2]上海中医药大学附属曙光医院

出　　处：《中西医结合心脑血管病杂志》2014年第2期205-207,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：河南中医学院博士基金项目(No.BSJJ2009-45);上海市市属博士学科点建设基金项目(No.20080246404)

摘　　要：目的观察潜阳合剂(QYHJ)对自发性高血压大鼠(SHR)胸主动脉血管重构的影响。方法将12周龄SHR随机分为QYHJ高、中、低剂量以及福辛普利组、SHR对照组,每组8只;另以8只同周龄雄性WKY大鼠为正常对照组。QYHJ高、中、低剂量组给药剂量分别为22.94g/kg、11.47g/kg和5.74g/kg,福辛普利组给药剂量为0.9mg/kg,连续给药8周。SHR对照组和正常对照组给予等容积双蒸水。至20周龄时采集胸主动脉标本,分别行苏木素伊红染色和Masson染色,测量各组大鼠胸主动脉重构指标。结果 SHR对照组胸主动脉血管外径(ED)、内径(LD)、中膜厚度(MT)、中膜厚度与内径比(MT/LD)、血管中膜横截面积(MCSA)、血管横截面积(CSA)、血管中膜横截面积与血管横截面积比(MCSA/CSA)、血管中膜胶原容积分数(CVF)值均较正常对照组增加(P<0.05或P<0.001),发生明显肥厚性重构。QYHJ高剂量组大鼠胸主动脉ED、LD、MCSA、CSA值较SHR对照组减小(P<0.05或P<0.01);MT值较SHR对照组有减小趋势,但差异无统计学意义;MT/LD、MCSA/CSA值与SHR对照组差异无统计学意义。SHR对照组胸主动脉血管中膜CVF值均较WKY大鼠增加(P<0.001);QYHJ高、中剂量组大鼠胸主动脉血管中膜CVF均较SHR对照组降低(P<0.05或P<0.01)。结论 QYHJ对SHR胸主动脉肥厚性重构具有一定改善作用。Objective To observe the effects of Qianyang Heji decoction （QYHJ）on the arterial remodeling of the thoracic aorta in spontaneously hypertensive rats（SHR）. Methods Forty 12 week - old male SHR were assigned to 5 groups randomly：SHR control group, SHR＋ Fosinopril, SHR-- QYHJ High, SHR＋ QYHJ Medium, SHR; QYHJ low. Each group consisted of 8 animals. 8 male WKY rats （12 week- old） were used as normal control group. SHR＋ QYHJ High, Medium and Low were given QYHJ contained crude drug of 22.94 g/kg,ll. 47 g/kg,5.74 g/kg respectively; SHR＋Fosinopril were given suspl of fosinopril of 0.9 mg/kg; SHR and WKY control group were given the distilled water of the same volume. Drugs were given for 8 weeks constantly. Thoracic aorta was harvested at 20 week- old. After hematoxylin and eosin staining, Masson＇ s trichrome staining, the values of arterial remodeling of the thoracic aorta of each group rats were measured. Results The values of ED,LD,MT,MT/LD,MCSA,CSA,MCSA/CSA of tho- racic aorta of SHR were increased compared with that of WKY （P;0.05 or P;0. 001）. Hypertrophic remodeling was developed on the thoracic aorta of SHR. The values of ED,LD,MCSA,CSA of SHR＋QYHJ High were reduced compared with that of SHR （P〈0.05 or P〈0.01）. The values of MT of SHR＋QYHJ High were reduced compared with that of SHR, but there was no statistic sig- nificance. The values of MT/LD and MCSA/CSA of SHR were not significantly different from that of three groups of SHR＋QYHJ. The values of CVF of SHR were increased compared with that of WKY （P〈0. 001）. The values of CVF of SHR＋QYHJ High and Medium were reduced compared with that of SHR （P〈0.05 or P〈0.01）. Conclusion QYHJ could improve hypertrophic remode- ling of thoracic aorta in SHR.

关 键 词：自发性高血压大鼠 潜阳合剂 血管重构 

分 类 号：R259[医药卫生—中西医结合]