醒脑静注射液对高血压脑梗死大鼠模型的神经保护作用  

Xingnaojing Injection Attenuates Neurologic Deficits Against Focal Ischemic Injury in Stroke-prone Renovascular Hypertensive Rats

作　　者：马斌[1] 刘璐[2] 张扬[1] 孙逸坤[1] 赵一舟[1] 高永红[1] 辛喜艳[1] 刘剑[1] 

机构地区：[1]北京中医药大学东直门医院 [2]首都医科大学附属北京中医医院

出　　处：《中西医结合心脑血管病杂志》2014年第2期212-214,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然基金项目(No.81173230);北京中医药大学校级课题

摘　　要：目的研究醒脑静注射液对大鼠高血压脑梗死的神经保护作用及其机制。方法采用双肾双夹肾血管进行高血压大鼠模型的制备,1个月后高血压大鼠模型制备成功,将动物随机分成3组,即假手术组、模型组、醒脑静给药组。然后采用改良的ZeaLonga法(改良的线栓法)制备大鼠大脑中动脉局灶性脑缺血损伤模型,醒脑静给药组于缺血造模后4h腹腔注射醒脑静注射液(3mL/kg),每隔30min给药1次,共给药3次。假手术组和模型组于造模后30min给予等体积的生理盐水腹腔注射1次。造模后24h,采用Zea-Longa方法对大鼠进行神经功能缺损评分后,测定脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)活性。结果脑缺血24h后,模型组大鼠神经功能评分与假手术组相比明显增高,醒脑静给药组较模型组显著降低,差异有统计学意义(P<0.01)。脑缺血后,模型组大鼠缺血脑组织中SOD、GSH-Px和MDA的活性较假手术组显著降低(P<0.05)。醒脑静给药组SOD、GSH-Px活性均高于模型组(P<0.05或P<0.01),MDA活性显著低于模型组(P<0.05)。结论醒脑静注射液能减轻神经损伤症状,降低脑组织MDA活性。增高脑组织中SOD、GSH-Px活性,能减轻脑缺血损伤,其机制可能与拮抗氧自由基损伤有关。Objective To elucidate the neuroprotective effect and the molecular mechanisms of Xingnaojing injection against focal ischemic injury in stroke - prone renovascular hypertensive rats （RHRSP） rats. Methods The RHRSP rats were used to create per- manent middle cerebral artery occlusion （pMCAO） model. Xingnaojing injection was given 3 mL/kg per 30 min for 3 times,starting 4 h after pMCAO. The superoxide dismutase （SOD）,glutathione peroxidase （GSH- PX）,and the content of malondialdehyde （MDA） in brain tissue were measured. Results Reduced level of MDA, obviously elevated activities of SOD and GSH - Px were found in Xingnaojing injection treated groups. Conclusion The findings suggested that Xingnaojing injection neuroprotection was associated with its anti - oxidative property in focal cerebral ischemia in the RHRSP rats. Administration of Xingnaojing injection at an early phase could significantly attenuate ischemia to induce brain injury,and its mechanism of action might be via anti- oxidative pathway.

关 键 词：高血压 脑梗死 醒脑静注射液 神经保护作用 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：R285.6[医药卫生—中药学]