血塞通注射液对局灶性脑梗死大鼠不同恢复时点大脑皮层Syp和PSD-95蛋白表达的影响  

Effect of Xuesaitong Injection on PSD-95 and Syp Protein Expression in Cortex of Focal Cerebral Infarction Rats

作　　者：刘丽星[1] 朱陵群[1] 刘炜[1] 吴建华[1] 邹忆怀[2] 陈家旭[3] 赵歆[3] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室和北京市重点实验室 [2]北京中医药大学东直门医院 [3]北京中医药大学基础医学院

出　　处：《中西医结合心脑血管病杂志》2014年第2期217-219,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然科学基金资助项目(No.30772803;81173235);北京中医药大学创新团队资助项目(No.2011CXTD-07)

摘　　要：目的观察血塞通注射液(主要成分为三七总皂苷)在不同时间点对局灶性脑梗死大鼠皮层部位突触后致密物质(post sypaptic Density-95,PSD-95)及突触素(synaptophysin,Syp)蛋白表达的影响,探索三七总皂苷对脑缺血受损神经元重塑的作用机制。方法采用改良Longa方法制备局灶性脑梗死模型,将40只SD大鼠随机等分为假手术组、模型组、三七总皂苷小剂量组、三七总皂苷大剂量组、尼莫地平组。各组动物分别于术后3d、7d、14d、28d麻醉断头取材,行免疫组织化学检测PSD-95及Syp蛋白的表达。结果模型组PSD-95蛋白表达在术后3d及7d明显降低,14d逐渐恢复正常,到28d时表达量较同时间点假手术组增高(P<0.01);Syp蛋白在术后模型组表达亦明显降低(P<0.01),术后14d及28d,三七总皂苷组的表达较模型组显著升高(P<0.01)。结论三七总皂苷可以促进局灶性脑梗死后PSD及Syp蛋白表达,从而促进神经重塑与修复。Objective To observe the effect of Xuesaitong Injection on PSD- 95 and Syp protein after Middle cerebral artery occlu- sion（MCAO）operation. Methods Healthy mature male SD rats were randomly divided into model group,normal saline group,Xue- saitong small -dose group,Xuesaitong high -dose group, positive control group （nimodipine group）. Each group was executed at 3 days, 7 days,4 days and 28 days and then the brain tissue was taken out to observe the expression changes of PSD- 95 and Syp by im- munohistochemistry. Results The expression of PSD- 95 in the model group was decreased at 3 and 7 days after the MCAO opera- tion,and gradually came to the normal level at 14 days （P（0.01）. Syp expression also decreased obviously in model group. The ex- pression in each treatment group was higher than that in the model group. At 14th and 28th day,the expression in Xuesaitong high- dose group was significantly increased compared with that in the model group （P（0.01）. Conclusion Xuesaitong Injection could in- crease the expression of PSD - 95 and Syp protein so as to promote nerve regeneration.

关 键 词：局灶性脑梗死 突触后致密物质 突触素 血塞通注射液 

分 类 号：R285.5[医药卫生—中药学]