3种方法检测弓形虫IgG抗体的效能  

Efficiency of three methods for detecting Toxoplasma IgG antibody

作　　者：杨亚晓 陈玉昆 魏世锦 宋任浩[2] 

机构地区：[1]河北省柏乡县人民医院检验科,柏乡055450 [2]河北省血液中心检验科

出　　处：《中国血吸虫病防治杂志》2014年第1期109-110,共2页Chinese Journal of Schistosomiasis Control

摘　　要：目的比较3种不同的检验方法对弓形虫IgG抗体的检测效能,并进行分析。方法采用临床上常用的金标法、间接血凝试验(IHA)、酶联免疫吸附实验(ELISA),对304份血清标本进行弓形虫IgG抗体平行检测,比较各检测方法的敏感性、特异性和Youden指数。结果金标法、IHA、ELISA这3种方法对弓形虫IgG抗体检测的敏感性分别为85.5%、89.8%和91.9%,三者之间差异没有统计学意义(χ2=4.12,P>0.05);特异性分别是92.4%、96.6%和97.5%,差异亦无统计学意义(χ2=4.06,P>0.05)。ELISA法的检测效率和Youden指数分别为94.1%和0.89,高于IHA法和金标法。结论 ELISA法检测弓形虫IgG抗体的敏感性和特异性较高,可自动化,适合于大规模的弓形虫IgG抗体筛查。Objective To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody. Methods To tally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method, indirect hemagglutina tion test （IHA）, and enzyme-linked immunosorbent assay （ELISA）, and the sensitivity, specificity and Youden index of these methods were compared. Results The detection sensitivities of gold marked method, IHA, and ELISA for Toxoplasma IgG anti body were 85.5%, 89.8% and 91.9% respectively （ X^2 = 4.12, P 〉 0.05） ; the specificities were 92.4%, 96.6% and 97.5% respec tively （ X^2= 4.06, P 〉 0.05）. The detection efficiency and Youden index of ELISA were 94.1% and 0.89 respectively, being high er than those of IHA and gold marked method. Conclusion The sensitivity and specificity of the ELISA method for Toxoplasma IgG antibody are higher, and in addition, it can be automated. Therefore, it is suitable for large-scale Toxoptasma IgG antibody screening.

关 键 词：弓形虫 抗体 IGG 金标法 间接血凝试验 酶联免疫吸附实验 

分 类 号：R531.8[医药卫生—内科学]