Na^+转运蛋白SKC1基因转化大豆的研究  

Genetic Transformation of Na^+ Transporter Gene SKC1 into Soybean Mediated with Agrobacterium

作　　者：于志晶[1] 蔡勤安[1] 刘艳芝[1] 齐广勋[1] 马瑞[1] 董英山[1] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033

出　　处：《吉林农业科学》2014年第1期1-5,共5页Journal of　Jilin Agricultural Sciences

基　　金：转基因生物新品种培育重大专项(2011ZX08004-002)

摘　　要：本研究构建了水稻Na+转运蛋白SKC1基因植物表达载体pTF-SKC1,标记基因为Bar基因。以子叶节为外植体,利用农杆菌介导法将SKC1导入大豆中。经过除草剂抗性筛选后获得的再生植株经PCR方法鉴定,转基因植株阳性率为50%,初步证明SKC1基因已整合到大豆基因组中。耐盐性试验结果表明,转基因植株的耐盐性高于对照。In this study the plant expression vector pTF-SKC1, harboring a Na＋ transporter gene SKC1 which is derived from rice and a selection marker gene bar, was constructed. The gene was transformed into soybean mediated with Agrobacterium tumefaciens by using cotyledon node as explants. PPT resistant regenerated plants were identified by PCR, and the rate of positive plants was 50%. The test for salt-tolerance of the transformed plants showed that over-expressing of SKC1 in soybean could enhance salt tolerance of the transgenic plant of soybean.

关 键 词：SKC1基因 遗传转化 大豆 

分 类 号：S565.101[农业科学—作物学]