丹参注射液对慢性输卵管炎性大鼠TGF-β_1、Ⅰ型胶原的影响研究  

Study of the effect of Danshen injection on type I collagen and TGF-β_1 in rats with chronic salpingitis

作　　者：谷风[1] 

机构地区：[1]广州中医药大学附属清远中医院,广东清远511500

出　　处：《现代中西医结合杂志》2014年第8期802-804,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：广东省中医药管理局课题(20132063)

摘　　要：目的观察丹参注射液对慢性输卵管炎性大鼠转化生长因子β1(TGF-β1)、Ⅰ型胶原表达的影响,阐明其作用机制。方法 SPF级SD雌性大鼠,随机抽取10只为正常组,其余采用"苯酚胶浆法"制备慢性输卵管炎性大鼠模型,并随机分为模型组、西药组、丹高组、丹中组。造模8 d后,灌胃给药,1次/d,连续21 d。第22天,腹腔注射麻醉后,取大鼠输卵管组织,免疫组化法测定TGF-β1、Ⅰ型胶原的表达。结果输卵管组织细胞胞浆、间质细胞中见黄染颗粒,模型组最多,西药组较多,丹高组和丹中组明显减少,但丹中组优于丹高组。结论丹参注射液能够降低TGF-β1、Ⅰ型胶原的异常表达,可抗组织粘连及纤维化,故用于治疗输卵管阻塞性不孕具有良好的临床疗效。Objective It is to observe the effect of Danshen injection on type I collagen and TGF-β1 in the rats with chronic salpingitis, and to discuss its mechanism. Methods SPF level female SD rats were selected in which 10 rats were randomly chosen as as the normal group, and the others were prepared for the chronic salpingitis model by ＂ phenol glue method＂ ,and then randomly divided into model group, western medicine group, high dose of Danshen injection group and middle close of Danshen injection group. In eight days after model establish, the rats were given drugs by intragastric administration once a day and for 21 days. On the 22 day, oviduct tissue of the rats were gotten after anesthesia by intraperitoneal injection to detect the expressions of TGF-β1 and I collagen by immunohistochemical determination. Results The yellow stained particles in the oviduct tissue cytoplasm and stromal cells were found, and the quantity was the most in the model group, more in the western medicine group, much less in Danshen injection groups, but the effects were more better in high dose of Danshen injection group than that in middle dose of Danshen injection group. Conclusion Danshen injection can reduce the abnormal expressions of TGF-β1 and I collagen, has anti-tissue adhesions and fibrosis effects, thus it has good clinical efficacy on tubal obstruction infertility.

关 键 词：慢性输卵管炎 丹参注射液 大鼠 TGF-β1、Ⅰ型胶原 

分 类 号：R332[医药卫生—人体生理学]