创伤失血性休克大鼠肠系膜淋巴液对血管内皮细胞程序性坏死的影响  

Effect of mesenteric lymph on vascular endothelial cell necroptosis of rats with trauma-hemorrhagic shock

作　　者：王林林[1] 王兵[2] 于金宝[1] 张立亚[1] 王玉亮[3] 崔尧丽[2] 王勇强[2] 

机构地区：[1]天津医科大学一中心临床学院,天津300070 [2]天津市第一中心医院重症医学科天津市急救医学研究所 [3]卫生部危重病急救医学重点实验室

出　　处：《山东医药》2014年第8期5-8,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81301624);国家临床重点专科建设项目(2011-873)

摘　　要：目的观察创伤失血性休克(THS)大鼠肠系膜淋巴液对血管内皮细胞程序性坏死的影响。方法建立THS大鼠模型,收集THS手术(T/HS)及假手术(T/SS)大鼠肠系膜淋巴液;将传代的人脐静脉内皮细胞(HUVECs)分为3组,T/HS、T/SS组分别加入1∶1稀释的T/HS、T/SS大鼠肠系膜淋巴液,对照组加PBS;培养3 h后,MTT法检测细胞活性,Western blot法检测程序性坏死的特异性蛋白受体交互作用蛋白1(RIP1)、RIP3。将HUVECS分为6组,A(D)、B(E)、C(F)组分别加入PBS、细胞程序性坏死阻滞剂Nec-1、溶媒DMSO预处理1 h,D、E、F组分别加入1∶1稀释的T/HS大鼠肠淋巴液。孵育3 h后,观察细胞活性及细胞程序性坏死情况。结果与T/SS组、对照组比较,T/HS组细胞活性降低、RIP1及RIP3表达量增加(P均<0.01);与D、F组比较,E组细胞活性增加、RIP1和RIP3表达量降低(P均<0.01);与A、B、C组比较,E组细胞活性降低、RIP1和RIP3表达量增加(P均<0.01)。结论 THS肠淋巴液能够导致血管内皮细胞程序性坏死,该作用能被Nec-1部分抑制。Objective To observe the influence of mesenteric lymph on the vascular endothelial cell necmptosis of rats with trauma-hemorrhagic shock （THS）.Methods We constructed THS mt models and collected mesenteric lymph from trauma-hemorrhagic shock （T/HS） rats and trauma-sham shock （T/SS） rats.The subcultured human umbilical vein endothelial cells（HUVECs） were divided into 3 groups.T/HS group was cultivated with T/HS mesenteric lymph and T/SS group was cultivated with T/SS mesenteric lymph （all mesenteric lymph were diluted 1∶1 with PBS）,and the control group was cultivated with the same amount PBS.After being cultured for 3 h,the cell viability was measured by MTT,and receptor interaction protein 1 （RIP1） and RIP3 were analyzed by Western blot.The subcultured HUVECs were divided into 6 groups,PBS,Nec-1,solvent DMSO were respectively added into A （D）,B （E）,C （F）groups,1 hour later,T/HS mesenteric lymph that diluted 1 ∶ 1 with PBS was added into D,E and F groups.After 3 h incubation,the cell activity and necroptosis was observed.Results The cell viability was lower,and the expression levels of RIP1 and RLP3 were increased in T/HS group as compared with those of the control group and T/SS group （all P ≤ 0.01）.The cell viability was higher,and the expression levels of RIP1 and RIP3 were decreased in the E group as compared with those of D and F groups （all P ≤0.01）,but they were respectively lower and increased as compared with A,B and C groups （all P ≤0.01）.Condusion Mesenteric lymph of rats with THS can induce vascular endothelial cell necroptosis,and Nec-1 can inhibit the occurrence of necroptosis partially.

关 键 词：创伤失血性休克 程序性坏死 阻滞剂 受体交互作用蛋白1 人脐静脉内皮细胞 多器官功能障碍综合征 急性肺损伤 急性呼吸窘迫综合征 大鼠 

分 类 号：R441.9[医药卫生—诊断学]