转PEPC基因水稻的抗逆性研究  

Studies on Stress Resistance of PEPC Transgenic Rice

作　　者：李万昌[1] 师学珍[1] 张文国 王静[1] 王丹[1] 姬生栋[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]封丘县农业局,河南封丘453300

出　　处：《河南农业科学》2014年第2期28-30,共3页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关项目(102102310318)

摘　　要：为了研究转C4光合基因水稻的抗逆性,以稳定的转磷酸烯醇式丙酮酸羧化酶(PEPC)基因水稻和未转基因水稻(对照)为供试材料,研究其在干旱、高温、高光等逆境条件下的光合速率及抗氧化酶(SOD、POD)活性变化。结果表明:在干旱和高温、高光条件下,转PEPC基因水稻和对照光合速率较正常水分条件下均下降,但对照分别下降39.1%和30.4%,转PEPC基因水稻只下降8.0%和12.0%,说明转PEPC基因水稻具有较稳定的光合速率;超氧阴离子(O-·2)产生速率均增加,对照增加的速度远快于转PEPC基因水稻,转PEPC基因水稻分别增加2.25倍和0.45倍,对照却相应增加3.73倍和2.10倍;对照中SOD、POD活性几乎没有变化,而转PEPC基因水稻中SOD、POD活性增强,比正常条件下分别增加0.34倍和1.20倍,说明转PEPC基因水稻清除O-·2能力增强,从而可有效减轻膜脂过氧化程度。In order to elucidate the mechanism of photoprotection in PEPC transgenic plants, the net photosynthetic rate（Pn）, the endogenous superoxide anion（O2-） generation rate, and the variation of superoxide dismutase（SOD） and peroxidase （POD） activities in leaves were measured under the stress conditions of drought, high temperature and high light intensity. The results showed that the PEPC transgenie plants exhibited higher Pn and activities of SOD and POD than the untransformed ones, less generation rate of O2-. It was suggested that the photo-oxidation tolerat;ce of PEPC transgenic rice was higher than the untransformed rice.

关 键 词：水稻 PEPC基因 抗逆性 酶活 

分 类 号：S511.032[农业科学—作物学]