慢性HBV感染者不同免疫状态证候虚实分类与T、B及NK细胞亚群的相关性研究  

The TCM syndrome classification of different immune status of chronic HBV infection and T,B and NK cells subgroup correlation studies

作　　者：张兵[1] 杨丽莎[2] 唐美媛[3] 张敏[1] 聂琳[1] 

机构地区：[1]桂林医学院附属医院消化内科,广西桂林541001 [2]桂林医学院附属医院中医科,广西桂林541001 [3]桂林医学院附属医院检验科,广西桂林541001

出　　处：《重庆医学》2014年第6期641-643,647,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81260602);广西中医药管理局中医药科技专项课题立项资助项目(GZKZ09-85)

摘　　要：目的了解慢性乙型肝炎病毒(HBV)感染者不同免疫状态证候虚实分类与T、B细胞亚群及自然杀伤(NK)细胞之间的相互关系。方法将3种不同免疫状态的慢性HBV感染者分为虚证组、实证组,检测其外周血淋巴细胞亚群和NK细胞,并对上述各组指标进行比较分析。结果在3种不同免疫状态中,所有虚实两组患者的CD4+T细胞百分比均较对照组(健康献血员)低(P<0.05),其中在免疫耐受状态中实证组CD4+T细胞低于虚证组(P<0.05);在免疫清除状态中,CD8+T细胞百分比由高至低依次为实证组、对照组、虚证组(P<0.05)。3种不同免疫状态中,实证组CD4+/CD8+比值均显著低于虚证组或对照组,在免疫清除状态中虚实两组的B淋巴细胞百分比高于对照组,NK细胞低于对照组(P<0.05)。结论慢性HBV感染者存在着免疫功能紊乱;HBV感染不同免疫状态证候虚实分类与T、B细胞亚群及NK细胞之间存在一定相关性,免疫功能检测对于虚实证型的判定具有一定的临床应用价值。Objective To investigate the different immune status of TCM classification and the levels of T and B cells and the re-lationship between the NK cells .Methods Three different immune state of patients with chronic HBV were divided into asthenia syndrome（AS） group and sthenia syndrome（SS） group ,and then we detected the peripheral blood lymphocyte subsets and NK cells ,and compared the above each index analysis .Results In three different immune status ,all patients with AS and SS groups CD4＋ T cell percentages are lower than normal control group （P control group〉 the AS group （P〈0 .05） .Three different immune status ,the ratio of CD4＋ /CD8＋ SS group were significantly lower than the AS group or control group ,in a state of immune clearance the SS and the AS group B lymphocyte percentage were higher than the control group ,the percentage of NK cells was lower than the control group （P〈0 .05） .Conclusion Chronic HBV infection have immune dysfunction ;HBV infected different immune status of TCM classification have relationship with T and B cell subsets and NK cells ,and the immune function test has certain clinical application value for the judgment of TCM syndrome type .

关 键 词：T淋巴细胞 B淋巴细胞 杀伤细胞 天然 慢性乙型肝炎 免疫状态 中医证型分类 

分 类 号：R512.62[医药卫生—内科学]