冷胁迫处理下冬小麦东农冬麦1号叶片蛋白质的差异表达分析  

Analysis of expressed differential proteins in leaf of winter wheat Dongnongdongmai 1 under cold stress

作　　者：苍晶[1] 韩瑞[1] 于晶[1] 徐庆华[1] 赫福霞[1] 李怀伟[1] 赵浡彤 张克俭[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2014年第2期1-4,共4页Journal of Northeast Agricultural University

基　　金：高等学校博士学科点专项科研基金项目(20112325110003);国家自然科学基金人才培养能力提高科研训练项目(J1210069);黑龙江省教育厅科学技术研究项目;东北农业大学大学生创新训练项目

摘　　要：以冬小麦东农冬麦1号为试验材料,将三叶期麦苗经4℃冷胁迫处理7 d后提取叶片蛋白(25℃处理为对照组),利用双向电泳(2-DE)技术分析差异表达蛋白。所得双向电泳图谱经ImageMaster 2D Platinum 7软件分析检测到26个差异表达蛋白质点,选取其中3个进行质谱(MALDI-TOF-MS)分析,所得肽段序列经数据库搜索比对获得的匹配蛋白有3-磷酸甘油醛脱氢酶、ATP酶和Rubisco活化酶。这3种蛋白可能通过提高植物的光合能力、促进植物体内糖分的积累、改善植物对水合离子吸收等方面参与植物的抗冷机制。Total proteins from the leaves of winter wheat Dongnongdongmai 1 were extracted when seedlings at three-leaf period treated at 4 ℃ cold stress for seven days （seedlings treated at 25 ℃ as con- trol）, and differentially expressed proteins were analyzed by two-dimensional electrophoresis （2-DE）. The 2-DE maps obtained were analyzed by ImageMaster 2D Platinum 7 software and 26 differentially expressed protein spots were detected, 3 spots were selected for MALDI-TOF-MS analysis and peptide fragment se- quences were searched in the database which were matched to glyceraldehyde-3-phosphate dehydroge- nase, ATPase and Rubisco activase. These three proteins might involved in the mechanisms of cold resis- tance through enhancing the photosynthetic capacity, promoting the accumulation of sugar contents and im- proving the absorption of hydrated ion in plants and so on.

关 键 词：冬小麦 冷胁迫 双向电泳 差异蛋白 

分 类 号：S767.5[农业科学—森林保护学] X172[农业科学—林学]