超临界流体技术制备姜黄素脂质体的工艺考察  

Optimization of technology for supercritical fluid technology in preparation of curcumin liposome

作　　者：张志丽[1] 张志云[1] 张维[1] 周丽莉[1] 

机构地区：[1]沈阳药科大学制药工程学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2014年第2期81-86,93,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的采用响应面法优化超临界流体技术制备姜黄素脂质体的处方工艺。方法运用中心复合设计考察磷脂质量浓度、脂药质量比和pH值三因素对姜黄素脂质体包封率的影响及其交互作用。根据实验数据建立二次回归模型,并进行响应面分析。结果超临界流体技术制备姜黄素脂质体的工艺可行,且最佳处方配比为:磷脂质量浓度51.16 g·L-1、脂药质量比24.04∶1、pH值6.28。结论该条件下姜黄素脂质体包封率为80.43%,与模型预测值79.55%偏差较小,说明预测模型可靠性较高,可用于处方工艺的优化。Objective To optimize supercritical fluid technology by response surface methodology for prepar- ing curcumin liposome. Methods The effects and interaction of three factors on encapsulation efficiency of curcumin liposome,such as SPC concentration,soy phosphatidyl choline （SPC）/curcumin （Cur）, and pH, were investigated by the method of central composite design. Quadratic regression models were set up ac- cording to the experimental data. Response surfaces were delineated on the basis of best fit mathematic mod- els. Results Using supercritical fluid technology to prepare curcumin liposome was feasible. The optimal technology conditions of curcumin liposome were as follows： the SPC concentration was 51.16 g. L-1, the ratio of SPC/Cur was 24. 04：1 and pH was 6. 28. Conclusions The results show that the encapsulation effi- ciency of the curcumin liposome is 80. 43 % under the optimal conditions. And there exists small deviation between the measured value of index and model predicted value of 79.55%. It shows that the response sur- face analytical method is reliable and can be used in the process of prescription optimization.

关 键 词：超临界流体技术 姜黄素 脂质体 响应面法 

分 类 号：R94[医药卫生—药剂学]