HPLC法同时测定菊花中绿原酸、黄芩苷和槲皮素的含量  

Determination of the contents of chlorogenic acid,baicalin and quercetin in the Chrysanthemi Flos by HPLC

作　　者：袁琦[1] 赵辉[1] 蒲晓辉[1] 刘闪闪[1] 孙娜[1] 覃江克[2] 

机构地区：[1]河南大学药学院,河南开封475001 [2]广西师范大学化学化工学院,广西桂林541004

出　　处：《沈阳药科大学学报》2014年第2期112-115,共4页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金资助项目(21002015)

摘　　要：目的建立高效液相色谱法同时测定菊花中绿原酸、黄芩苷和槲皮素的含量。方法采用Hypersil ODSC18柱（4．6mm×250mm，5μm），甲醇-体积分数0．4％乙酸溶液为流动相，梯度洗脱，流速1．0mL·min-1，检测波长为337nm，柱温25℃。结果绿原酸、黄芩苷和槲皮素均能达到基线分离，且有较宽的线性范围和良好的线性关系（r〉0．9995）；回收率均在99．2％-99．4％内。结论该方法简便准确，稳定好，可用于同时测定菊花中绿原酸、黄芩苷和槲皮素的含量。Objective To establish an HPLC method for the determination of chlorogenic acid, baicalin, and quercetin in the Chrysanthemi Flos. Methods A hypersil ODS C18 column （ 4.6mm×250mm,5μm） was used with methanol-0. 4% acetic acid solution as mobile phase. Gradient elution programme was ap- plied with the flow rate of 1.0 mL. min-1. The detection wave length was set at 337 nm and the column temperature was 25 ℃. Results Chlorogenic acid, baicalin and quercetin were separated perfectly with wide linear range and good linear relationship （ r 〉 0. 999 5 ） ; average recovery were 99.2%-- 99.4%. Conclusions This method is simple and accurate, and the results are stable. It can be used to determine the contents of chlorogenic acid,baicalin and quercetin in Chrysanthemi Flos.

关 键 词：高效液相色谱法 绿原酸 黄芩苷 槲皮素 菊花 含量测定 

分 类 号：R917[医药卫生—药物分析学]