过氧化氢氧化亚甲基蓝褪色光度法测定痕量铜  

Determination of Trace Copper by Catalytic Kinetic Spectrophotometry Based on H202 Oxidizing Methylene Blue

作　　者：景彩霞[1] 柴红梅[2] 

机构地区：[1]延安大学医学院,陕西省延安市光华路38号716000 [2]延安大学化学与化工学院,陕西省延安市圣地路580号716000

出　　处：《光谱实验室》2014年第2期246-249,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：延安大学自然科学重点基金项目（YDZ2012-09）

摘　　要：在NH3-NH4Cl缓冲溶液中，Cu2＋对H2O2氧化亚甲基蓝的褪色反应具有强烈的催化作用，据此建立了催化动力学光度法测定痕量铜的新方法。该方法的线性范围为0．001-0．013μg／mL，检出限为6．03×10-4μg／mL。对0．007μg／mL的Cu2＋进行11次平行测定的相对标准偏差为1．5％。本该方法用于环境水样（山泉水、雨水和自来水）中痕量铜的测定，回收率为98．9％-100．2％。A new catalytic kinetics spectrophotometic method was developed for the determination of trace Cu2＋. The method was based on the catalytic effect of Cu2＋ on the oxidation of methylene blue by H2O2 in a buffer solution of NH3-NH4C1. The linear range of this method was 0.001--0.013μg/mL and the detection limit was 6.03×10^-4μg/mL. The relatively standard deviations were 1.5% on parallel 11 times of determinations to 0.007μg/mL Cu2＋. The method has been applied to the determination of trace Cu2＋ in water samples with satisfactory results and the range of recovery is 98.9%--100.2 %.

关 键 词：催化动力学分光光度法 铜 亚甲基蓝 

分 类 号：O657.32[理学—分析化学]