苦马豆素人工抗原接种和田羊的免疫学研究  

Immunological study on the Hetian sheep inculated with artificial antigen of swainsonine

作　　者：王帅[1,2] 胡建军[1,2] 陈根元[2] 贾琦珍[2] 马春晖[2,3] 张玲[1,2] 

机构地区：[1]塔里木大学动物科学学院,新疆阿拉尔843300 [2]新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300 [3]石河子大学动物科技学院,新疆石河子832000

出　　处：《黑龙江畜牧兽医》2014年第3期21-24,28,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31160497);新疆生产建设兵团博士资金专项(2008JC07);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题项目(HS201207)

摘　　要：为了探索苦马豆素(SW)人工抗原SW-BSA免疫接种后对和田羊的保护作用,试验通过间接血凝试验(IHA)和间接酶联免疫吸附试验(ELISA)分析SW-BSA免疫和田羊后血清抗体效价的变化,并检测试验羊血清E-玫瑰花环率的变化,以及血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、α-甘露糖苷酶(AMA)、肌酐(CRE)活性的变化,同时检测试验组尿液和血液中有无SW。结果表明:SW-BSA可以诱导和田羊产生高效价的抗SW抗体,而且这种高效价保持的时间较长,机体E-玫瑰花环率显著上升;试验组与对照组比较,血清中AST、ALT、AKP、LDH、AMA、CRE水平均差异不显著(P>0.05),尿液和血液中均未检查出SW。说明合成的人工抗原SW-BSA具有较好的免疫原性和安全性。To explore the protective effect of artificial antigen for swainsonine （SW -BsA） in Hetian sheep after immune incubation, the chan- ges of the titers of the antibody in sreum were after immunization in Hetian sheep with SW - BSA analyzed using indirect hemagglutiuation （IHA） and enzyme linked immunosorbent assay （ ELISA）. The change of E - rosette formation rate was detected, and then the changes of the activities of aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, alkaline phosphatase （AKP）, lactate dehydrogenase （LDH） , ct - mannosidase （AMA） , and creatinine （CRE） in serum were analyzed, and swainsouine in the urine and blood was also detected in the experimental groups. The result showed that SW - BSA could induce Hetian sheep to secrete high - titer antibody against swainsonine, and the high titer could maintain a long time, while the E - rosette formation rate was significantly increased in the body. There were no significant differences （P 〉0.05） on the serum levels of AST, ALT, AKP, LDH, AMA, and CRE between the test groups and control group, and swainsonine was not detected in the urine and blood. The results indicate that the synthesized artificial antigen of SW - BSA has good immunogenicity and safety.

关 键 词：苦马豆素(SW) 人工抗原 免疫 和田羊 间接血凝试验(IHA) 间接酶联免疫吸附试验(ELISA) 疯草中毒 生化指标 

分 类 号：S852.4[农业科学—基础兽医学]