关于毒氟磷安全性评价的彗星试验分析  

Study on the comet assay analysis for the safety assessment of dufulin

作　　者：何琦[1] 卢黎[1] 杨红[1] 张勇[1] 

机构地区：[1]贵州大学动物科学学院/高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵阳550025

出　　处：《黑龙江畜牧兽医》2014年第3期29-31,208,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：贵州省农业科技攻关项目[黔科合NY(2007)字(3007)号];贵州省自然科学基金项目[黔科合J字(2008)2128];贵州大学青年科技基金项目[贵大自青基合字(2007)074]

摘　　要：为了研究毒氟磷致小鼠遗传毒性,试验将小鼠外周血淋巴细胞悬液随机分成5组,第1组为D-Hank’s阴性对照组;第2,3,4组为试验组,分别用30%毒氟磷可湿性粉剂按1 mg/L、5 mg/L、10 mg/L剂量进行体外染毒;第5组为10μmol/L阿霉素阳性对照组。通过MTT法测定毒氟磷对细胞的增殖抑制作用,并对DNA损伤进行彗星试验(SCGE)分析。结果表明:1 mg/L、5 mg/L、10 mg/L毒氟磷对细胞的抑制率分别为(6.751±0.021)%、(11.521±0.009)%、(16.254±0.022)%;5 mg/L毒氟磷可致小鼠外周血淋巴细胞DNA损伤,随着染毒剂量的增加DNA受损程度加剧,呈现剂量-效应关系。To study genetic toxicity of dufulin on peripheral blood lymphocyte in mice, peripheral lymphocyte suspensions of mice were randomly divided into 5 groups. The first was D - Hank＇ s blank control group; the second, third and fourth were test groups, respectively. The peripher- al blood lymphoeytes were exposed in vitro to the 30% dufulin wet table powder at concentrations of 1 mg/L, 5 mg/L, and 10 rag/L, ~spec- tively. The fifth group was a positive control group with 10 I^mol/L of doxorubicin. The inhibition effect of dufulin on the cells was measured by MTI＂ assay, and the comet assay （single cell gel eleetrophoresis） was used to detect the DNA damage. The results showed that the inhibition rates of dufulin at concentrations of 1 mg,/L, 5 mg/L and 10 mg/L on the cells were （ 6. 751 ± 0. 021 ） % , （ 11. 521 ± 0. 009 ） %, and （16. 254 ±0.022）%, respectively . Dufulin at the concentration of 5 mg/L could cause DNA damage in peripheral blood lymphocytes in mice. And with the increase of dose, DNA damage aggravated, showing a dose - effect relationship.

关 键 词：彗星试验 外周血淋巴细胞 剂量一效应 30%毒氟磷可湿性粉剂 小鼠 MTT法 氟中毒 

分 类 号：S859.83[农业科学—临床兽医学]