克雷伯氏菌生产絮凝剂M-C11的培养优化及其在污泥脱水中的应用  

Optimized Cultivation of a Bioflocculant M-C11 Produced by Klebsiella pneumoniae and Its Application in Sludge Dewatering

作　　者：刘杰伟[1] 马俊伟[1] 刘彦忠[1] 杨娅[1] 岳东北[2] 王洪涛[2] 

机构地区：[1]北京师范大学环境学院,环境模拟与污染控制国家重点联合实验室,北京100875 [2]清华大学环境学院,北京100084

出　　处：《环境科学》2014年第3期1183-1190,共8页Environmental Science

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07313-002)

摘　　要：从活性污泥中筛选分离得到可产生微生物絮凝剂的克雷伯氏菌C11，对微生物絮凝剂M—C11的培养基碳源、氮源、无机盐等条件进行优化．探讨将微生物絮凝剂应用于活性污泥脱水时，pH、CaCl：投加量、M—C11投加量等因素对污泥脱水效果的影响，并与常规化学絮凝剂的调理效果对比分析．结果表明，絮凝剂M—C11生产的最优培养条件：30g·L^-1葡萄糖、2g·L^-1NaNO，、0．5g·L^-1MgSO。分别作培养基碳源、氮源、无机盐，在37℃，150r·min^-1振荡培养48h，絮凝活性高达91．70％．M．C11在pH为4-8、温度为20-60％的范围内具有良好的絮凝稳定性．克雷伯氏菌产絮凝剂M·C11应用于调理污泥脱水，在pH为6、3mLM-C1l、4mLCaCl，（1％）的最佳投加组合下，调理后污泥比阻（SRF）和含水率分别由原泥的11．64×10^12m·kg^-1和98．86％，降低至4．66×10^12m·kg^-1和83．74％，调理后的污泥脱水效果显著优于硫酸铝、聚合氯化铝（PAC）等无机絮凝剂．微生物絮凝剂具有成本低廉、易生物降解、无二次污染等优点，且对污泥pH、盐度等条件具有良好的适应性，可作为新型高效的污泥脱水调理剂．A bioflocculant-producing Klebsiella pneumoniae strain Cllwas screened out from activated sludge and the optimal medium conditions for the production o＇f microbial floceulant M-C11 were determined. The bioflocculant was used in activated sludge dewatering and compared with conventional chemical conditioners. Effects of pH, CaC12 dosages and M-Cll dosages on sludge dewaterability were investigated. The optimized conditions for M-Cll production indicated that the optimal medium carbon, nitrogen, metal ion were 30 g.L-1 glucose, 2 g.L-l NaNO3 and 0. 5 g.L-1 MgSQ, respectively. The flocculating rate with kaolin suspension was as high as 91.70% , when incubated in a rotary shaker at 150 r.min-~ and 37~C for 48 h. The microbial focculant showed excellent pH and thermal stability over a pH range of 4-8 and a temperature range of 20-60~C. Then the bioflocculant M-Cll produced by Klebsiella pneumoniae was employed to enhance the sludge dewaterability. The sludge resistance to filtration （SRF） and cake moisture decreased from 11.64 x 1012m.kg-1 and 98.86% to 4. 66 x 10^12m.kg-1 and 83.74% , respectively. Sludge dewatering performance was more significantly improved with the optimal conditioning dosages （pH = 6, 3 mL M-C11, 4 mL CaC1z ）, than inorganic flocculating reagents such as aluminum sulfate and polymeric aluminum chloride （PAC）. The microbial flocculant has advantages over traditional sludge conditioners for its lower cost, benign biodegradability and ignorable secondary pollution. In addition, it was favorably adapted to the sludge pH and salinity. The novel bioflocculant could be used as a potential conditioner for sludge dewatering.

关 键 词：微生物絮凝剂 克雷伯氏菌 优化培养 絮凝稳定性 污泥脱水 

分 类 号：X703.1[环境科学与工程—环境工程]