桥本甲状腺炎患者杀伤细胞免疫球蛋白样受体与人类白细胞抗原基因对频率的分析  

Analysis of killer cell immunoglobulin-like receptor and human leukocyte antigen gene-pair frequencies in Hashimoto's thyroiditis patients

作　　者：李建婷[1] 郭成[1] 侯岩峰[1] 焦玉莲[1] 赵跃然[1] 孙慧[1] 徐进[1] 曹铭锋[1] 高聆[1] 赵家军[1] 张海清[1] 李明龙[1] 

机构地区：[1]山东大学附属省立医院内分泌科,济南250021

出　　处：《中国免疫学杂志》2014年第1期110-113,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(30901461)

摘　　要：目的:探讨杀伤细胞免疫球蛋白样受体(Kill cell immunoglobulin-like receptor,KIR)与人类白细胞抗原(Human leukocyte antigen,HLA)组成的受体-配体基因对与桥本甲状腺炎(Hashimoto’s thyroiditis,HT)的关联性。方法:选择散发HT患者44例和健康对照43例,提取全血基因组DNA,序列特异性引物聚合酶链反应检测4个KIR基因和8个HLA-C基因,根据受体-配体特异识别原则组成KIR2DL1+HLA-C2、KIR2DL2/2DL3+HLA-C1、KIR2DS1+HLA-C2和KIR2DS2+HLA-C1共4对KIR/HLA-C基因对,分析各基因对频率在两组人群中的差别。结果:活化性KIR2DS2+HLA-C1基因对在HT患者组与对照组频率差异有显著性(36.36%vs 16.28%,P<0.05)。其余基因对在两组间未见明显差异。结论:HT患者活化性KIR/HLA-C基因对频率升高,传递活化性信号活化NK细胞和T细胞,可能是HT发病的免疫遗传学因素之一。Objective： More research have been carried out to demonstrate that the gene pair consisted by kill cell immunoglobulin-like receptor （KIR） and human leukocyte antigen （HLA） played a role in the pathogenesis of many immunologic diseases. The aim of this study is to investigate the relationship of certain KIR/HLA-C gene pair with Hashimoto＇ s thyroiditis （HT）. Methods： For- ty-four HT patients and forty-three randomly matched healthy controls were enrolled to detect the KIR and HLA-C genotypes. The ge- nomic DNA were extracted, followed by four selected KIR genes and eight HLA-C genes were detected, and all of the target genes were determined by a polymerase chain reaction using sequence-specific primers （PCR-SSP）. The four KIR genes were KIR2DL1, KIR2DL2/3, KIR2DS1 and KIR2DS2/3, as well as eight HLA-C consisted of HLA-Cw01-08 genes. Then the frequencies of four gene pairs, KIR2DL1 ＋ HLA-C2, KIR2DL2/3 ＋ HLA-C1, KIR2DS1 ＋ HLA-C2 and KIR2DS2 ＋ HLA-C1, were compared between HT patients and healthy control individuals. Results： The frequency of KIR2DS2/HLA-C1 gene pair was significantly higher of the patient group than that of the control group （36.36% vs 16.28% ,P 〈 0.05 ）. There were no significant differences in the frequencies of other KIR/HLA-C gene pairs. Conclusion： The increased frequencies of KIR2DS2 and HLA-C1 gene pair might confer to the pathogenesis of HT disease.

关 键 词：杀伤细胞免疫球蛋白样受体 人类白细胞抗原 基因 甲状腺炎 自身免疫性疾病 

分 类 号：R581[医药卫生—内分泌]