Toxicity of Five Herbs in Aconitum L. on Tetrahymena thermophila Based on Spectrum-effect Relationship  

作　　者：Xiao-jiao Sun Tian-tian Liu Yan-ling Zhao Wen-jun Zou Jia-bo Wang Shu-xian Liu Zhi-yong Sun Rui-sheng Li Xu Zhou Man Gong Rui-lin Wang 

机构地区：[1]College of Pharmacy, Chengdu University of Traditional Chinese Medicine [2]China Military Institute of Chinese Medicine, 302 Hospital of People's Liberation Army [3]Animal Laboratory Center, 302 Hospital of People's Liberation Army [4]Department of Integrative Medical Center, 302 Hospital of People's Liberation Army

出　　处：《Chinese Herbal Medicines》2014年第1期29-35,共7页中草药（英文版）

基　　金：State Natural Science Foundation(81173571);The"Twelfth Five-Year Plan"Foundation of China People’s Liberation Army(CWS11C164);The Major Projects of the National Science and Technology(2012ZX10005010-002-002)

摘　　要：Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.Objective To explore the active components with toxic effects in five Aconitum L. herbal medicines on Tetrahymena thermophila. Methods The fingerprints of five Aconitum L. herbal medicines were established by ultra-high performance liquid chromatography （UPLC） and the toxicity was evaluated by using a TAM Air Isothermal Calorimeter on Tetrahymena thermophila SB1 10. Results By analyzing the spectrum- effect relationships between UPLC fingerprints and toxic effects, the active components which had the toxic effects were obtained. Conclusion This work provides a general model of the combination of UPLC and microcalorimetry to study the spectrum-effect relationships of the five Aconitum L. herbal medicines, which could be used to evaluate the toxic effects and analyze the principal toxic components of the five Aconitum L. herbal medicines. On the whole, this result provides the experimental basis for the safe use of the five Aconitum L. herbal medicines in clinic.

关 键 词：Aconitum L. herbal medicines MICROCALORIMETRY spectrum-effect relationships Tetrahymena thermophila ultra-high performance liquid chromatography 

分 类 号：R285[医药卫生—中药学]