实时荧光定量PCR对乙肝DNA检测的影响因素  

Real-time Fluorescent Quantitative PCR for the Influence Factors of Hepatitis B DNA Testing

作　　者：赖新华[1] 吕娜[1] 彭艳辉[1] 

机构地区：[1]东莞市长安医院,广东东莞523852

出　　处：《中外医学研究》2014年第5期63-64,共2页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的:探讨实时荧光定量PCR对乙肝DNA检测的效果,对影响因素进行分析总结。方法:选择2010年1月-2012年1月笔者所在医院收治的300例乙肝病毒感染患者,收集其血标本,通过实时荧光定量PCR检测方法进行乙肝DNA检验,对检验结果与临床诊断结果进行对比分析,对影响检验结果的因素进行分析总结。结果:本组300例乙肝感染患者血标本中,采用实时荧光定量PCR对乙肝DNA检测结果与临床诊断结果相同276例(92.0%),漏诊误诊24例(8.0%),主要的影响因素包括实验室因素、标本因素和核酸提取方法因素等。结论:采用实时荧光定量PCR对乙肝DNA进行检测,具有较高的确诊率,但对检测结果产生影响的因素较多,需要加强注意,才能降低漏诊和误诊率,为治疗提供有效支持。Objective：To explore effect of real-time fluorescent quantitative PCR detection of hepatitis B DNA,analyze the influence factors.Method：From January 2010 to January 2012 were between 300 patients with hepatitis b virus （HBV） infection,collect the blood specimens,by real-time fluorescent quantitative PCR detection method for hepatitis B DNA testing,the result of the inspection result and clinical diagnosis,this paper compares and analyzes the effect factors of test results is analyzed.Result：300 cases of hepatitis b infection in patients with blood specimens,using real-time fluorescent quantitative PCR of hepatitis B DNA test results and clinical diagnosis results of 276 cases of same （92.0%）,misdiagnosis and misdiagnosed 24 cases,accounted for 8.0%,the main influence factors including laboratories,specimen and nucleic acid extraction method,etc.Conclusion：The real-time fluorescent quantitative PCR to detect HBV DNA,has higher diagnosis rate,but the influence on test results of many factors,the need to strengthen the attention,to decrease the rate of missed diagnosis and misdiagnosis,provide effective support for treatment.

关 键 词：实时荧光定量PCR 乙肝DNA 影响因素 

分 类 号：R512.62[医药卫生—内科学] R440[医药卫生—临床医学]