用标样比对法鉴定放线菌提取物中的主要活性成分  

Identifying the Main Active Compounds in Extracts of Some Actinomyces Fermentation Broth Using Standard Sample as Comparison

作　　者：万中义[1,2] 张亚妮[1,2] 张志刚[1,2] 王开梅[1,2] 杨自文[1,2] 

机构地区：[1]湖北省生物农药工程研究中心 [2]湖北省农业科技创新中心生物农药分中心,武汉430064

出　　处：《湖北农业科学》2013年第23期5760-5763,5766,共5页Hubei Agricultural Sciences

基　　金：国家公益性行业科研专项(200903052);湖北省农业科技创新中心项目(2013-620-008-001)

摘　　要：在新型农用抗生素的筛选工作中,对部分具有广谱抗真菌活性的放线菌提取物HPLC-MS检测数据进行分析后,得到紫外吸收及质谱碎片等光谱学信息,根据天然产物数据库比对结果,疑为环已酰亚胺。将环已酰亚胺标准样品在相同条件下分析,得到的紫外吸收光谱及质谱碎片信息与检测样品几乎完全一致。由此推断曾疑似未知的主要活性化合物为环已酰亚胺。采用该方法可快速准确地对活性化合物进行鉴定,从而大大提高筛选效率。In screening new agricultural antibiotics,a lot of extracts with wide spectrum and strong fungicidal activity were found and took as unknown compounds.Based on the UV spectrum and MS fragments,combining with database retrieval,these compounds seemed to be cycloheximide.Using standard sample as control,both of them were analyzed by HPLC-MS under the same parameter,the same UV and mass fragments was obtained.The active compounds were identified as cycloheximide.Using this method,many compounds can be rapidly and accurately identified.

关 键 词：农用抗生素 环已酰亚胺 标准样品 标样比对法 鉴定 

分 类 号：R979.1[医药卫生—药品]