胆固醇酯转运蛋白对3T3-L1脂肪细胞内胆固醇的影响  

Effect of CETP on lipid metabolism in 3T3-L1 cell lines

作　　者：陈晓丽 常毅娜[2] 朱晓慧 鞠现霞[3] 付真真 郭雯 高贝贝 符金香 周红文 

机构地区：[1]南京医科大学第一附属医院内分泌病科,江苏省210029 [2]河南科技大学第二附属医院内分泌病科 [3]泰安市中医医院超声科

出　　处：《江苏医药》2014年第4期385-387,F0003,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(81170747);江苏省六大人才高峰项目(2008-18D)

摘　　要：目的探讨胆固醇酯转运蛋白(CETP)对脂肪细胞脂代谢的影响。方法构建稳定表达CETP的单克隆小鼠3T3-L1脂肪前体细胞株,并通过有限稀释法和流式细胞术分选法获得不同CETP表达水平的3T3-L1细胞株加以鉴定,测定细胞内胆固醇含量。结果 pcDNA3.1-CETP-GFP重组质粒构建成功,建立并获得12株稳定表达不同CETP水平的3T3-L1细胞株,挑选出高克隆(克隆编号1)、中克隆(克隆编号6)、低克隆(克隆编号8)CETP表达水平的3T3-L1细胞株。胆固醇测定显示,表达CETP的3T3-L1细胞总胆固醇及游离胆固醇含量增加,并且胆固醇含量随着CETP的水平升高而升高(P<0.05或P<0.01)。结论 CETP可能通过调节脂肪细胞内胆固醇的含量从而影响葡萄糖代谢。Objective To investigate the effect of cholesterol ester transfer protein（CETP） on lipid metabolism in adipocyte 3T3-L1 cell lines. Methods The 3T3-L1 cells monoclonal strains expressing different levels of CETP gene in rats were constructed and identified by limiting dilution method and flow cytometry. The cholesterol content was measured. Results The 3T3-L1 cell lines with high, medium, low levels of CETP expression were picked out by RT-PCR. Contents of intracellular total cholesterol and free cholesterol were significantly higher in 3T3-L1 adipocytes expressing CETP（P〈0. 05 or P〈0. 01）,which increased as CETP level elevated. Conclusion CETP may affect glucose metabolism via the regulation of cholesterol content in adipocytes.

关 键 词：胆固醇酯转运蛋白 3T3-L1细胞 胆固醇 

分 类 号：R589[医药卫生—内分泌]