骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔胫骨牵张成骨的实验研究  

Experimental study of new bone formation after transplantation of autologous bone marrow mesenchymal stem cells overexpressing bone morphogenetic protein 2 in a rabbit tibia distraction model

作　　者：任志勇[1] 邱世超[1] 黄现峰[1] 

机构地区：[1]解放军第89医院全军创伤骨科研究所,山东省潍坊市261021

出　　处：《中国矫形外科杂志》2014年第5期453-457,共5页Orthopedic Journal of China

摘　　要：[目的]观察人骨形态发生蛋白2基因修饰的自体骨髓间充质干细胞移植对兔胫骨牵张成骨新骨形成的促进作用。[方法]48只新西兰白兔随机摸球法分为3组。建立牵张成骨动物模型,在固定期第2 d,实验组于牵张间隙注射人骨形态发生蛋白2基因修饰的自体骨髓间充质干细胞;对照组注射等量自体骨髓间充质干细胞;空白组不注射任何物质。[结果]在固定期2周及6周实验组牵张区在大体观察、HE染色、X线观察方面成骨质量均好于对照组和空白组。12周后取牵张成骨区标本作骨组织骨密度和生物力学测定,结果显示实验组新生骨质量较高,骨愈合情况要优于对照组和空白组。[结论]骨形态发生蛋白2基因修饰的自体骨髓间充质干细胞移植能有效提高兔胫骨牵张成骨新骨形成质量。[ Objective ] To investigate the effect of transplantation of autogenous bone marrow mesenehymal stem cells （BMSCs） overexpressing human bone morphogenetie protein （hBMP） -2 on osteogenesis in a rabbit shin bone distraction model using histomorphological methods. [ Methods ] Forty - eight New Zealand white rabbits were randomly divided into 3 groups of 16 rabbits each. On the second day of the consolidation period, the distraction gap was injected with hBMP -2 -trans- fected autogenous BMSCs, the same number of autogenous BMSCs, or nothing in the experimental group, control group, and blank group, respectively. [ Results ] The ossification quality was better in the experimental group than in the control group or the blank group by gross observation, hematoxylin and eosin staining, and radiographic examination after 2 and 6 weeks of con- solidation. After 12 weeks, specimens were collected from the areas of stretch ossification for measurement of bone mineral densi- ty and biomechanieal properties. The quality of the new bone and state of bone healing were better in the experimental group than in the control group or the blank group. [ Conclusion ] Transplantation of hBMP - 2 - transfected autogenous BMSCs can effec- tively promote bone regeneration in a rabbit shin bone distraction osteogenesis model.

关 键 词：骨形态发生蛋白2 基因修饰 骨髓间充质干细胞 牵张成骨 

分 类 号：R457.7[医药卫生—治疗学]