燃煤型地方性氟中毒病区人血浆H_2S、Hcy、VitB_6、VitB_(12)和叶酸水平的变化  

Changes of plasma levels of H_2S,Hcy,Vit B_6,Vit B_(12) and folic acid in subjects from the coal-burning endemic fluorosis area

作　　者：潘际刚[1,2] 吴昌学[3] 李毅[3] 张婵[3] 陈涛[2] 官志忠[1,3] 

机构地区：[1]贵阳医学院病理学教研室,贵州贵阳550004 [2]贵阳医学院生理学教研室,贵州贵阳550004 [3]贵阳医学院分子生物学重点实验室,贵州贵阳550004

出　　处：《中国药理学通报》2014年第2期250-252,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81160335);科技部国际合作项目(No 2013BAI05B03,2010DFB30530);贵州省国际科技合作计划(黔科合外G字[2011]7014号);贵州省科学技术厅贵阳医学院联合基金(黔科合LG字[2012]018号;黔科合计Z字[2012]4010)

摘　　要：目的观察燃煤型地方性氟病区人血浆硫化氢(H2S)及其代谢相关氨基酸的水平。方法采用离子选择电极、亚甲基蓝法和酶联免疫吸附法检测氟病区人血浆氟、H2S、同型半胱氨酸(Hcy)、VitB6、VitB12和叶酸(FA)的水平。结果与对照组相比,氟病区患者均有明显的氟斑牙,血浆氟含量明显增加(P<0.01),而H2S含量则明显降低(P<0.05);血浆Hcy、VitB6、VitB12和FA的水平在两组差异均无显著性(P>0.05)。结论燃煤型地方性氟中毒可明显降低人血浆H2S的水平,这可能与地氟病区人神经系统损伤有关。Aim To determine the plasma level of hy-drogen sulfide （H2S） and the level of H2S metabolism- related amino acids of individuals from the coal-burning endemic fluorosis area. Methods The plasma levels of fluorine, H2S, homocysteic acid （Hcy） , vitamin B6 （ Vit B6 ）, vitamin B12 （ Vit B12 ） and folic acid （FA） were detected by ion-selective electrode, methylene blue and enzyme linked immunosorbent assay, respec-tively. Results Compared with control, in addition to obvious dental fluorosis, fluorine concentration was in-creased significantly （ P 〈 O. 01 ）, whereas H2S con- centration was decreased in plasma of subjects from the fluorosis area （P 〈 0. 05 ）. There was no significant difference in plasma levels of Hcy, Vit B6, Vit B12 and FA between two groups （ P 〉 0.05 ）. Conclusion Endemic fluorosis can reduce plasma level of H2S and that could be associated with neural injuries of subjects from the coal-burning fluorosis area.

关 键 词：氟中毒 硫化氢 血浆 同型半胱氨酸 维生素B. 维生素B12 叶酸 

分 类 号：R12[医药卫生—环境卫生学] R446.11[医药卫生—公共卫生与预防医学]