鱼腥草地下茎提取物诱导胃癌细胞SGC-7901凋亡机制的研究  

Mechanism of SGC-7901 apoptosis induced by Houttuynia cordata thunb subterraneous stem extraction

作　　者：刘金娟[1] 杨成流 陈永强[1] 蒋继宏[1] 

机构地区：[1]江苏师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《中国药理学通报》2014年第2期257-261,共5页Chinese Pharmacological Bulletin

基　　金：江苏师范大学博士学位教师科研支持项目(10XLR25);江苏省高校自然科学基金资助项目(11KJB310011)

摘　　要：目的对鱼腥草不同部位提取物诱导SGC-7901细胞凋亡及其机制进行研究。方法将鱼腥草地上茎(AS)、地下茎(SS)、叶(L)的粉末经乙醇提取得浸膏;处理SGC-7901细胞48 h后,采用Alamar blue法检测其对SGC-7901细胞增殖的影响;筛选出较高活性浸膏,光学显微镜、Hoechst33258染色观察细胞形态;DNA Ladder琼脂糖凝胶电泳检测细胞凋亡情况;Caspase-3活性检测试剂盒测定Caspase-3酶活性。Western blot检测凋亡相关因子的表达;Caspase-9抑制剂(Z-LEHD-FMK)验证浸膏调控的信号转导通路。结果各部位浸膏对SGC-7901细胞均有一定的增殖抑制活性,其中SS提取物活性最强;不同浓度的SS提取物作用SGC-7901细胞48 h后,细胞明显凋亡;DNA ladder条带也明显;Caspase-3的活性明显高于对照组;Bax、Bid、Bak、p53、Caspase-9表达上调,Bcl-2表达下调;Caspase-9抑制剂(ZLEHD-FMK)能够逆转SS对SGC-7901细胞的增殖抑制活性。结论 SS对SGC-7901细胞的增殖抑制作用最强,且是通过Caspase-9介导的线粒体凋亡信号转导通路来调控细胞凋亡的。Aim To investigate the apoptosis of SGC-7901 cells induced by Houttuynia cordata extraction and its possible underlying mechanisms. Methods The powder of Houttuynia cordata＇s aerial stem（AS） , subterraneous stem （ SS ） , leaves （ L ） was extracted with ethanol. The proliferation of SGC-7901 cells in-duced by extractions was examined by Alamar blue as- say. The inverted microscope and Hoechst 33258 stainning were used to detect the morphological chan-ges. The apoptosis of SGC-7901 cell was evaluated by DNA ladder. The protein expression was measured by Caspase-3 activity kit and Western blot. The specific inhibitor of Caspase-9 （Z-LEHD-FMK） was used to validate the signal transduction pathway. Results Allextractions showed the inhibited activity of SGC-7901 cells in a dose-dependent manner, especially the SS extraction. The expression of Bax, Bak, Bid, p53, Caspase-9 was upregulated, and Bcl-2 was downregu- lated. Z-LEHD-FMK significantly increased the cell vi- ability of SGC-7901 cells induced by SS. Conclusions The inhibited activity is different for different extrac-tions of each part of the Houttuynia cordata thunb, and SS is the highest. SS induces the apoptosis of SGC-7901 cells through the mitochondria-mediated pathway.

关 键 词：鱼腥草 地下茎提取物 SGC-7901细胞 凋亡 CASPASE-9 分子机制 

分 类 号：R284.1[医药卫生—中药学] R329.24[医药卫生—中医学]