RAGE和NF-κB及p21在小鼠螺旋神经节细胞的表达研究  

RAGE,NF-κB,p21 expressions in mouse spiral ganglion cells

作　　者：高险亭 林昶[2] 卢岭 梁耕田 陈志宏[2] 章许晶 

机构地区：[1]武汉市第三医院耳鼻咽喉头颈外科,武汉430000 [2]福建医科大学附属第一医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第4期265-268,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：武汉市卫计委临床医学科研项目(No:WX13B09)

摘　　要：目的:通过研究晚期糖基化终产物受体(RAGE)、核转录因子NF-κB、p21在幼、老年C57BL/6j小鼠螺旋神经节细胞(SGC)的表达,探讨老年性聋的发病机制。方法:将C57BL/6j小鼠分为2月龄、10月龄组各25只,分别行组织学切片观察耳蜗SGC形态结构,免疫组织化学检测RAGE、NF-κB、p21在SGC中的表达,利用IPP6图像分析软件计算平均光密度值,值越大,表达量越多。结果:①10月龄小鼠SGC数目明显比2月龄少,分别为(20±6)个和(39±5)个,差异有统计学意义(P<0.01);②RAGE在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.179±0.025和0.308±0.050;NF-κB在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.181±0.045和0.335±0.120;p21在2月龄和10月龄小鼠耳蜗SGC平均光密度值分别为0.160±0.023和0.365±0.031,两组分别比较,差异均有统计学意义(P<0.05)。结论:RAGE、NF-κB、p21在SGC中有表达且随年龄增大表达增多,推测RAGE、NF-κB、p21可能参与老年性聋的发病过程。Objective：To research advanced glycation end-product receptors（RAGE）, NF-kB,p21 expressions in C57BL/6] mice cochlea spiral ganglion cells（SGC） ,and then to investigate the presbyacusa pathogenesis. Meth-od：To take C57BL/6J mice： 2 month 25,and 10 month 25. Histological sections were observed the SGC. RAGE, NF-kB, p21 were immunohistochemical in the SGC,with IPP6 to IOD. Result： O The count SGCs of 10 month-old was obviously decreased comparing to 2 month-old, the count of 2 month SGC is 39± 5,10 month group is 20±6, P〈0.01 ;（2）RAGE, NF-kB ,p21 expressed in spiral ganglion cell,different place with different age,and the means optical density in the 10 month are higher than the 2 month, respectively. The IOD of RAGE expression in 2 month SGC：0. 179±0. 025,10 month IOD：0. 308±0. 050;The TOD of NF-kB expression in 2 month SGC：0. 181± 0. 045,10 month IOD：0. 335±0. 120;The IOD of p21 expression in 2 month SGC：0. 160±0. 023,10 month IOD： 0. 365±0. 031, compare with 2 group, respectively＇, P〈O. 05, and the difference has statistics sense. Conclusion： RAGE,NF-kB,p21 expressions are in SGCs and increases with the aging of SGCs, suppose RAGE, NF-kB, p21 may participate in the process of preshyacusa pathogenesis.

关 键 词：老年性聋 晚期糖基化终产物受体 核转录因子 细胞周期抑制蛋白 螺旋神经节细胞 

分 类 号：R764.43[医药卫生—耳鼻咽喉科]