不育温度下油菜温敏雄性不育系SSH文库的构建  

SSH library Construction of Brassica juncea L. Thermo-sensitive Male-sterile Line at Sterile Temperature

作　　者：叶晓明[1] 彭少丹[2] 陈升位[1] 陆娜[1] 林良斌[1] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学农科专业实验教学中心,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2014年第1期6-10,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(31160289)

摘　　要：以温敏雄性不育系K121S在可育温度（10℃）下和不育温度（26℃）下三核期花蕾为材料,以可育温度下的cDNA为驱动子,通过抑制性差减杂交技术构建了不育温度下抑制性差减杂交（SSH）文库,其插入片段长度分布在200～1 000 bp左右,并随机挑选97个阳性菌落测序,得到20个表达序列标签（EST）,包括与ATP合酶β亚基、Rubp羧化酶小亚基同源的EST等,为进一步获得育性转换相关基因和揭示K121S的育性转换分子机理奠定了基础.In this study, using flower-bud at thinucleate stage from thermo-sensitive male-sterile line K121S （Brassica juncea） at fertile temperature and sterile temperature as material, SSH library was constructed with driver cDNA obtained at male-fertile temperature. Ninety-seven clones were chosen randomly in sequencing. Twenty EST were discovered analogous to genes such as ATP synthetase sub- unit beta, ribulose-1, 5-bisphosphate carboxylase small subunit. The size of the DNA inserted ranges from 200 to 1 000 bp. The library laid the foundation of obtaining of the genes for fertility conversion and clarification of molecular mechanism of K121S fertility conversion.

关 键 词：油菜 温敏雄性不育 抑制性差减杂交 文库 

分 类 号：S634.302.2[农业科学—蔬菜学]