云南蓝莓枝干溃疡病病原菌鉴定及rDNA-ITS序列分析  

Identification and the Internal Transcribed Spacer Regions Analysis of the Pathogen Causing Stem Canker on Blueberry in Yunnan

作　　者：余磊[1] 唐旭兵 赵建荣[1] RARISARA Impaprasert 徐胜光[1] 吴旭[1] 孔垂思[4] 

机构地区：[1]昆明学院农学院,云南省高校都市型现代农业工程研究中心,云南昆明650214 [2]云南烟草保山香料烟公司,云南保山678000 [3]朱拉隆功大学食品技术学院,泰国曼谷103303 [4]云南省农业科学院农业环境资源研究所,云南昆明650205

出　　处：《云南农业大学学报（自然科学版）》2014年第1期27-31,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(2011Z032);云南省科技厅应用基础研究(面上项目)(2011FZ180;2011FZ178);昆明学院科学研究基金项目(YJL11002)

摘　　要：自2010年云南省昆明市周边及丽江市蓝莓商业化种植区发生了一种蓝莓新病害,典型症状为茎干和枝条枯萎死亡,发病早期部分枝条和叶片枯萎但不脱落,韧皮或木质部横切面呈轻棕黄色或灰黑色变色.采用温室接种致病性测定、形态及分子鉴定等方法对蓝莓枝干溃疡病病原进行研究.结果表明：从各种植区采集病样15份,分离获得来自蓝莓病株茎干、枝等器官的15个生长较一致的真菌分离物;致病性试验证实,均为蓝莓枝干溃疡病病原菌;经形态学鉴定为Botryosphaeria dothidea,利用ITSI-5.8S-ITS4进行PCR扩增,并将测序结果在GenBank中进行同源性比对分析显示,其序列分析结果与B.dothidea的同源性为99％ ～100％,分子鉴定与形态学鉴定结果一致.该病原菌在蓝莓果树上的危害在云南省内尚属首次报道.The identification of the pathogens isolated from the stem blight on Highbush Blueberries （ Vaccinium corymbosum L. ） was carried out. Fifteen of diseased samples were collected and observed in Kunming and Lijiang in Yunnan Province （south-western China）. Typical symptoms of the disease were blight and dieback on the stems with lesions extending along entire branches. All of isolates were isolated from stem and twigs of infected blueberry plants. Based on the pathogenicity testing, the re-suhs proved that LIJIANG09 were the pathogens of highbush Blueberries dieback disease. The morpho- logical identification showed that LIJIANG09 was identified as Botryosphaeria dothidea. Further, mor- phological identification result was confirmed by analyzing the rDNA internal transcribed spacer region （ITSI-5.8S-ITS4）. BLAST searches at GenBank showed a high identity with B. dothidea reference se- quences （ITS： 〉 99% ）. Representative sequences of isolates from both regions were deposited in GenBank （ ITS ： Accession No. JX096631 ）. Morphological and molecular identification confirmed this fungal pathogen as B. dothidea. B. dothidea was first reported as the pathogen of Highbush Blueberries stem blight disease in Yunnan.

关 键 词：蓝莓 葡萄座腔菌 分子鉴定 

分 类 号：S436.639[农业科学—农业昆虫与害虫防治]