失血性贫血小鼠骨髓间充质干细胞向成骨成脂分化的变化  

Osteogenic and Adipogenic Differentiation of Bone Marrow Multipotent Mesenchymal Stem Cells in the Mice with Hemorrhagic Anemia

作　　者：吴梦青[1,2] 朱榕嘉[1] 刘开彦[1] 

机构地区：[1]北京大学人民医院,北京100044 [2]北京积水潭医院,北京100035

出　　处：《中国实验血液学杂志》2014年第1期177-182,共6页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(81270572)

摘　　要：本研究旨在检测骨髓间充质干细胞(multipotent mesenchymal stem cells,MSC)向成骨、成脂分化的变化,探讨骨髓间充质干细胞的分化状态对骨髓造血功能的影响。先对实验小鼠每隔1-2 d行内眦静脉取血0.3 ml,4周构建贫血模型,通过检测贫血小鼠外周血常规指标、网织红细胞比例、骨髓细胞造血集落形成以验证小鼠模型。通过检测贫血模型小鼠成纤维细胞集落形成、骨髓细胞和脂肪细胞的数量,并用实时定量PCR的方法检测骨髓成骨、成脂分化相关基因的表达来研究骨髓MSC分化潜能的改变。结果显示,与对照组相比,贫血小鼠红细胞数量和血红蛋白水平明显下降,网织红细胞比例增高,骨髓细胞造血集落形成单位数量增多;骨髓造血细胞增多,脂肪细胞减少;骨髓成纤维细胞集落形成单位增多并显著向成骨细胞分化;骨髓成骨分化相关基因Runx2和OSX的表达明显升高,而成脂相关基因aP2、PPARγ2的表达明显降低。结论:在骨髓造血功能活跃期小鼠骨髓间充质干细胞向成骨细胞分化能力增强,向脂肪细胞分化能力减弱。This study was aimed to investigate the effects of osteogenic and adipogenic differentiation of bone marrow multipotent mesenchymal stem cells （MSC）on hematopoiesis.A hemorrhagic anemia mouse model was established by exsanguinating of 0.3 ml blood from angular vein every 1-2 days for 4 weeks.The number of leukocytes,erythrocytes and neutrophils,hemoglobin level,ratio of reticulocyte in peripheral blood and bone marrow cell colony forming unit （CFU） were detected for the identification of the model.The differentiatial potential of MSC in the hemorrhagic anemia mice were identified by CFU-F,histopathologic analysis,and osteogenesis and adipogenesisrelated gene expression.The results showed that the erythrocyte numbers of peripheral blood and hemoglobin level decreased in the hemorrhagic anemia mice compared with the control,while the ratio of reticulocyte,the numbers of bone marrow cells and the CFU increased.Furthermore,the numbers of CFU-F,bone marrow hematopoietic cells,and osteogenic cells increased.However,the number of adipocytes decreased.Expressions of osteogenesis-related genes Runx2 and OSX were up-regulated,and adipogenesis-related genes aP2 and PPARγ2 were down-regulated in the hemorrhagic anemia mice compared with the control.It is concluded that the potential of osteogenic differentiation of MSC is enhanced,while the potential of adipogenic differentiation of MSC is weakened in the hemorrhagic anemia mice.

关 键 词：间充质干细胞 脂肪细胞 成骨细胞 失血性贫血 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]