TLR2和TLR4激动剂对人骨髓来源的间充质干细胞迁移能力的影响  

Influence of TLR2 and TLR4 Agonists on Migration of Human Bone Marrow Mesenchymal Stem Cells

作　　者：颜宗海 王兴兵[1] 汪健[1] 李来玲[1] 朱云霞[1] 

机构地区：[1]安徽医科大学附属省立医院血液科,合肥230001

出　　处：《中国实验血液学杂志》2014年第1期183-186,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(81270573);安徽省级高校自然科学基金(KJ2012Z188)

摘　　要：本研究探讨Toll样受体2(Toll-like receptors 2,TLR2)和TLR4激动剂对人骨髓来源的间充质干细胞(mesenchymal stem cells,MSC)迁移能力的影响及其机制。采用流式细胞术检测MSC表面TLR2和TLR4的表达情况,应用趋化实验和黏附实验观察PAM3CSK4(TLR2激动剂)和LPS(TLR4激动剂)对人骨髓来源的MSC的趋化活性和黏附活性的影响。结果表明,人骨髓来源的MSC表面表达TLR2(24.5±3.2)%和TLR4(91.3±5.2)%。与对照组相比,PAM3CSK4可明显抑制SDF-1诱导的MSC的趋化作用,并增强MSC的黏附作用;而LPS对MSC的趋化和黏附能力均无明显影响。进一步研究显示,PAM3CSK4可呈剂量依赖性抑制MSC的自发迁移作用,但对MSC表面CXCR4的表达无明显影响。结论:TLR2的活化可显著抑制MSC的迁移能力,这可能与其抑制MSC的自发迁移和增强MSC的黏附作用具有一定的关系。This study was aimed to investigate the influence of TLR2 and TLR4 agonists on the migration and adhesion activity of human bone marrow-derived mesenchymal stem cells （MSC） and to clarify the underlying mechanisms.The expression of TLR2 and TLR4 on MSC was detected by flow cytometry.The effects of TLR2 agonist （PAM3CSK4） and TLR2 agonist （LPS） on MSC migration and adhesion ability were evaluated with chemotaxis and adhesion test.The results indicated that expressive levels of TLR2 and TLR4 on surface of human bone marrow MSC were （24.5 ± 3.2） ％ and （91.3 ± 5.2） ％ respectively.Compared with the control group,the migration activity of MSC toward SDF-1 was decreased significantly in PAM3CSK4 group,while the adhesion activity of MSC was promoted by PAM3CSK4 exposure.However,both the migration activity toward SDF-1 and the adhesion activity of MSC were not changed significantly in LPS-treated group.Further,it was found that PAM3CSK4 did not affect the expressive level of CXCR4 on MSC,however,it could inhibit the spontaneous migration of MSC in dose dependent manner.It is concluded that activation of TLR2 can decrease the migration ability of MSC,which may associate with the decreased spontaneous migration ability and the increased adhesion activity of MSC.

关 键 词：TLR2 TLR4 MSC 迁移能力 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]