可视性观测造血干细胞归巢的研究进展  

Research Progress on Visual Observations of Hematopoietic Stem Cell Homing——Review

作　　者：吴梦瑶[1] 陈彤[1] 

机构地区：[1]复旦大学附属华山医院血液科,上海200040

出　　处：《中国实验血液学杂志》2014年第1期209-212,共4页Journal of Experimental Hematology

基　　金：国家重大研究计划(2011CB910404);国家自然科学基金项目(81070399)

摘　　要：造血干细胞移植(hematopoietic stem cell transplantation,HSCT)是治疗血液病、恶性肿瘤、某些遗传性疾病和自身免疫性疾病的重要手段。植入的造血干细胞(hematopoietic stem cell,HSC)能否顺利归巢至骨髓并重建造血是HSCT成功的关键。伴随着对HSC归巢机制的不断认知,人们已不再满足于纯粹的体外功能学研究,如何实现造血干细胞归巢的可视性观测、了解植入细胞在骨髓微环境中的定植程序成为研究者们迫切希望解决的问题。近年来,随着成像技术的发展,激光扫描共聚焦显微镜(confocal laser scanning microscope,CLSM)和双光子显微镜(two-photon microscope)能够对组织或细胞进行三维重建和实时观测,使可视性研究HSC归巢成为现实。本文对可视性研究方法及其在HSC归巢研究中的应用做一综述。Hematopoietic stem cell transplantation （HSCT） is an important mean for clinical treatment to many of hematological diseases,malignant diseases,hereditary diseases and autoimmune diseases.Whether the implanted hematopoietic stem cells （HSC） can home to bone marrow （BM） smoothly and reconstitute the hematopoiesis is the key to successful HSCT.With the cognition of HSC homing mechanism,the visual observation of HSC homing to BM is attracting more and more attention and helps to clarify the micro-dialogue between HSC and BM microenvironment.In recent years,with the development of imaging technology,confocal laser scanning microscope （CLSM） and two-photon microscope are able to make 3D reconstruction and real-time observation of the tissue or cells.Researches on HSC homing process visibily become reality.In this article the methods of visual research and their application in HSC homing observation are reviewed.

关 键 词：造血干细胞 归巢 共聚焦显微镜 

分 类 号：R457.7[医药卫生—治疗学] R329.28[医药卫生—临床医学]