Analysis of endophytic fungi in roots of Santalum album Linn.and its host plant Kuhnia rosmarinifolia Vent  

檀香及其宿主植物假蒿根中内生真菌的研究(英文)

作　　者：Si-sheng SUN Xiao-mei CHEN Shun-xing GUO 

机构地区：[1]Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2014年第2期109-115,共7页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Mega-Project for Innovative Drugs(No.2012ZX09301002-001-032);the National Natural Science Foundation of China(No.31170016)

摘　　要：Santalum album Linn. is an evergreen and hemi-parasitic tree, the heartwood-sandalwood of which was used during a long history in traditional Chinese medicine. Kuhnia rosmarinifolia Vent. is a good host for 1- or 2-year-old growing S. album. The interaction between S. album and K. rosmarinifolia is still little known. Many studies have been carried out on a number of plants for identification and diversity of endophytes. In this study, in total 25 taxa of endophytic fungi were isolated from the roots of S. album and the roots of K. rosmarinifolia. The most frequently isolated genera were Penicillium sp. 1 and Fusarium sp. 1 in the roots of S. album and K. rosmadnifolia, respectively. S. album is a root parasite of K. rosmarinifolia. The interesting result is that they apparently do not share the same endophytic fungi isolates. This study for the first time explored the content of endophytic fungi from S. album and K. rosmarinifolia, which provides important information for further studies.研究目的:檀香(Santalum album Linn.)为檀香科(Santalaceae)檀香属(Santalum)树种,属于常绿半寄生小乔木,其心材为传统名贵中药。檀香与其寄主植物假蒿通过根端的吸盘相连,探讨檀香与其宿主之间内生真菌的关系具有重要意义。创新要点:假蒿(Kuhnia rosmarinifolia Vent.)是幼龄檀香很好的寄主。目前已经对许多药用植物的内生真菌的分离和鉴定进行了大量研究,但是关于檀香和假蒿之间关系的研究报道较少。本研究首次探讨了檀香及其宿主植物假蒿根中内生真菌的数量和种类,为进一步研究两者之间的关系提供一些有用的信息。研究方法:采用组织块分离法从檀香的根和其宿主植物假蒿的根部材料分离内生真菌,并采用形态学鉴定和分子鉴定手段对分离的内生真菌进行鉴定。重要结论:从檀香的根和其宿主植物假蒿的根中160个组织块中分离和鉴定了共25个真菌类群,其中青霉属真菌Penicillium sp.1为檀香根中优势菌,镰孢属真菌Fusarium sp.1为假蒿根中优势菌。特别有意思的是两种植物根中没有分离到明显相同的内生真菌。

关 键 词：Endophytic fungi Santalum album Kuhnia rosmarinifolia Penicillium sp. Fusarium sp. 

分 类 号：S792.99[农业科学—林木遗传育种]