In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel of tea(Camellia sinensis L.)  

茶果皮多糖理化性质、体外抗氧化活性及对α-葡萄糖苷酶的抑制作用(英文)

作　　者：Yue-fei WANG Jie WANG Jing WU Ping XU Yi-qi WANG Jun-jie GAO Danielle HOCHSTETTER 

机构地区：[1]Department of Tea Science, Zhejiang University [2]Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture [3]College of Pharmacy, Zhejiang Chinese Medical University

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2014年第2期173-180,共8页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Ministry of Science and Technology of China(No.2012BAD36B06-5)

摘　　要：The conditions for extracting polysaccharides from tea （Camellia sinensis L.） fruit peel （TFPPs） were studied. Three parameters （temperature, time, and liquid/solid ratio） affecting the extraction of TFPP were optimized using response surface methodology （RSM）. Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of frac- tionated TFPPs （TFPP-0, TFPP-20, TFPP-40, and TFPP-60） were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only re- markable scavenging activity on 2,2＇-azinobis（3-ethylbenzothiazoline-6-sulfonic acid） diammonium salt （ABTS） and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.研究目的:利用响应面优化茶果皮多糖(TFPP)提取条件,用乙醇分级分段得到4个多糖组分(TFPP-0、TFPP-20、TFPP-40和TFPP-60),并研究其理化性质、抗氧化活性和对α-葡萄糖苷酶抑制作用,为综合高效利用茶果皮多糖资源提供理论基础。创新要点:1.首次将茶果皮作为一种潜在生物资源研究;2.首次研究茶果皮多糖这一功能成分;3.将工艺优化、理化性质和生物活性结合研究。研究方法:三因素三水平响应面设计(见表1),傅里叶转换红外光谱法分析茶果皮粗多糖的功能团结构(见图3),高效液相色谱法检测单糖组分(见表2),2,2'-氨基-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基清除法(见图4a)和铁离子还原能力法(FRAP)(见图4b)分析茶果皮多糖抗氧化活性。重要结论:1.茶果皮多糖是一种水溶性的酸性杂多糖蛋白复合物;2.乙醇分级是一种有效多糖分离手段;3.茶果皮多糖具有出色的生物活性;4.茶果皮资源可以作为一种可再生生物资源进行深度的开发。

关 键 词：POLYSACCHARIDES Tea （Camelfia sinensis L ） fruit peel Physicochemical properties Antioxidant activity α-Glucosidase inhibition 

分 类 号：R285[医药卫生—中药学]