Co-activation of nAChR and mGluR induces V oscillation in rat medial septum diagonal band of Broca slices  

作　　者：Ya-li WANG Jian-gang WANG Gao-xiang OU-YANG Xiao-li Li Zaineb HENDERSON Cheng-biao LU 

机构地区：[1]Department of Physiology and Neurobiology, Henan province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China [2]Department of pathophysiology Henan Province Key Laboratory of Brain Research, Xinxiang Medical University, Xinxiang 453003, China [3]National Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China [4]Insitute of Membrane and System biology, University of Leeds, Leeds, UK

出　　处：《Acta Pharmacologica Sinica》2014年第2期175-184,共10页中国药理学报（英文版）

摘　　要：Aim： To examine whether co-activation of nAChR and mGluR1 induced y oscillation （20-60 Hz） in rat medial septum diagonal band of Broca （MSDB） slices. Meth6ds： Rat brain sagittal slices containing the MSDB were prepared. Extracellular field potentials were recorded with glass microelectrodes. The nAChR and mGluR1 agonists were applied to the slices to induce network activity. Data analysis was performed off-line using software Spike 2. Results： Co-application of the nAChR agonist nicotine （1 pmol/L） and the mGluR1 agonist dihydroxyphenylglycine （DHPG, 25 pmol/L） was able to induce y oscillation in MSDB slices. The intensity of nAChR and mGluR1 activation was critical for induction of network oscillation at a low （8 oscillation） or high frequency （y oscillation）： co-application of low concentrations of the two agonists only increased the power and frequency of oscillation within the range of 8, whereas y oscillation mostly appeared when high concentrations of the two agonists were applied. Conclusion： Activation of mGluR1 and nAChR is able to program slow or fast network oscillation by altering the intensity of receptorAim： To examine whether co-activation of nAChR and mGluR1 induced y oscillation （20-60 Hz） in rat medial septum diagonal band of Broca （MSDB） slices. Meth6ds： Rat brain sagittal slices containing the MSDB were prepared. Extracellular field potentials were recorded with glass microelectrodes. The nAChR and mGluR1 agonists were applied to the slices to induce network activity. Data analysis was performed off-line using software Spike 2. Results： Co-application of the nAChR agonist nicotine （1 pmol/L） and the mGluR1 agonist dihydroxyphenylglycine （DHPG, 25 pmol/L） was able to induce y oscillation in MSDB slices. The intensity of nAChR and mGluR1 activation was critical for induction of network oscillation at a low （8 oscillation） or high frequency （y oscillation）： co-application of low concentrations of the two agonists only increased the power and frequency of oscillation within the range of 8, whereas y oscillation mostly appeared when high concentrations of the two agonists were applied. Conclusion： Activation of mGluR1 and nAChR is able to program slow or fast network oscillation by altering the intensity of receptor

关 键 词：NACHR MGLUR1 DHPG nicotine 8 oscillation y oscillation the medial septum diagonal band of Broca learning andmemory 

分 类 号：Q421[生物学—神经生物学] V212.12[生物学—生理学]