风湿性心脏病心房纤颤和非心房纤颤患者心房肌细胞内钙离子浓度变化  

Does Ca^(2+) overload exist in atrial myocytes of rheumatic heart disease patients with atrial fibrillation?

作　　者：周更须[1] 梁延春[2] 张荣庆[3] 贾国良[3] 蔡振杰[1] 

机构地区：[1]第四军医大学西京医院心脏外科,陕西西安710033 [2]沈阳军区总医院心脏内科,辽宁沈阳110015 [3]第四军医大学西京医院心脏内科,陕西西安710033

出　　处：《第四军医大学学报》2001年第2期138-140,共3页Journal of the Fourth Military Medical University

摘　　要：目的　观察风湿性心脏病 (RHD)心房纤颤 (AF)患者心房肌细胞内是否存在 Ca2 + 超载 .方法　急性分离 RHD伴 AF和非 AF患者的心房肌细胞 ,用 Fluo- 3作为钙指示剂 ,用共聚焦显微镜测定细胞内 Ca2 +浓度 .结果　 AF组心房肌细胞内 Ca2 +浓度明显高于非 AF组心房肌细胞内 Ca2 +浓度(5 17± 98) nmol· L- 1 vs(2 6 2± 6 5 ) nmol· L- 1 ,两组间差异显著 (P<0 .0 1) .结论　 RHD伴 AF患者心房肌细胞内存在Ca2AIM To observe whether Ca 2+ overload exists in atrial myocytes of rheumatic heart disease patients with atrial fibrillation. METHODS Atrial myocytes of rheumatic heart disease patients with and without atrial fibrillation were acutely isolated. Intracellular Ca 2+ concentration was mea-sured with the fluoresent Ca 2+ indicator Fluo-3 and laser scanning confocal microscopy. RESULTS Intracellular Ca 2+ concentration of atrial fibrillation group was significantly higher than that of non-atrial fibrillation group [(517±98) nmol·L -1 vs (262±65) nmol·L -1)]. CONCLUSION Ca 2+ overload exists in atrial myocytes of rheumatic heart disease with atrial fibrillation patients.

关 键 词：心房颤动 风湿性心脏病 心房肌细胞内 钙离子 

分 类 号：R541.2[医药卫生—心血管疾病] R541.75[医药卫生—内科学]