基于全身照射小鼠血清铜的生物剂量计的可行性研究  

Development of a new biodosimetry based on serum copper in γ-ray irradiated mice

作　　者：闵璇宇 张晓红[1] 娄志超 张亚男[1] 胡晓丹[1] 张海黔[1] 

机构地区：[1]南京航空航天大学,210016

出　　处：《中华放射医学与防护杂志》2014年第2期99-102,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：江苏省自然科学基金（BK20131355）;江苏省研究生教育创新资助项目（CXLX12-0145）

摘　　要：目的 探讨电离辐射全身照射小鼠后血清铜浓度的变化,研究作为生物剂量计的可行性.方法 昆明种小白鼠83只,按随机数字表法,根据照射剂量0、1、2、3、5 Gy分为对照组和照射组,再根据照射时间30 min、1、3、5、7、10、13、16 d共分为15个组,每组5只；另设8只双盲组,给予双盲剂量（2.0 Gy）照射.分别在照后30 min和7d,对不同剂量γ辐照小鼠进行眼眶取血,并通过5-Br-PADAP比色法检测小鼠的血清铜浓度.采用单因素方差分析法,比较不同剂量辐照小鼠血清铜浓度的差异；利用线性、二次线性拟合函数分析血清铜与γ辐射的剂量-效应关系.分别在照后30 min、1、3、5、7、10、13、16 d,检测受照剂量为2 Gy的小鼠血清铜浓度,以观测血清铜在照后的稳定性.采用双盲法依据得到的小鼠血清铜剂量-效应关系对双盲组小鼠的吸收剂量进行估算.结果 照后30 min、7d的不同剂量辐照小鼠血清铜浓度差异均有统计学意义（F=208.20、145.98,P＜0.05）,拟合得到的剂量-效应关系明显（r=0.989、0.995）.血清铜在照后30 min达到最低值,照后1～7d有所回升,7～14d维持平稳,14 d后继续回升.利用得到的剂量-效应关系所估算的小鼠生物剂量与双盲剂量（2 Gy）接近,照后30 min和7d估算值的95％置信区间分别为（1.83～2.25） Gy和（1.82～2.11） Gy.结论 基于血清铜的新型生物剂量计能够迅速、有效地评估小鼠的受照剂量.Objective To investigate the changes of serum copper in mice after whole-body irradiation and analyze the feasibility of these changes as a biological dosimeter.Methods Serum copper in mice exposed to 60 Co γ-rays（0,1,2,3,5 Gy） was collected from the orbital of mice and detected with 5-Br-PADAP colorimetric method at 30 min and 7 d after radiation.One-way analysis of variance was used to analyze the difference of serum copper in each group and Dunnett-t test was used to compare the difference between control group and irradiated groups.Linear and quadratic linear fitting function was used to analyze the relationship between serum copper and radiation dose.The change of serum copper was detected at 30 min,1,3,5,7,10,13 and 16 d after radiation to observe the persistence of serum copper.The established relationships were used to estimate the dose in 8 mice irradiated by a blind dose.Results The amount of serum copper in irradiated mice were significantly （F =208.20,145.98,P ＜ 0.05）dependent on the radiation doses with dose responses of y =-0.091x ＋ 0.936 and y =-0.011x2-0.032x ＋ 0.962 （r =0.989,0.995） at 30 min and 7 d post-irradiation,respectively.The concentration of serum copper at 2.0 Gy decreased at 30 min post-irradiation,increased at 1-7 d,then kept at a stable level at 7-14 d even increased slightly after 14 d.With these dose response curves,after radiation with a blind dose of 2 Gy,the absorb doses of mice were assessed to be （1.83-2.25） Gy and （1.82-2.11） Gy at 30 min and 7 d in 95％ confidence interval,respectively.Conclusions The serum copper is a quick,simple,and sensitive biomarker for the early assessment of absorb dose of irradiated mice.

关 键 词：生物剂量计 血清铜 Γ射线 小鼠 

分 类 号：R144[医药卫生—公共卫生与预防医学]