吲哚胺2,3-二氧化酶对小鼠同种异体肝细胞脾内移植的作用  

Effects of Indoleamine 2,3-dioxygenase on the Mice Allogeneic Hepatocytes Transplanted into Spleen

作　　者：严茂林[1] 刘一人[1] 曾凯[2] 王耀东[1] 田毅峰[1] 赖智德[1] 

机构地区：[1]福建医科大学省立临床医学院,福建省立医院肝胆外科,福州350001 [2]福建医科大学附属第一医院麻醉科,福州350005

出　　处：《福建医科大学学报》2013年第6期354-357,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2013J0271)

摘　　要：目的研究携带吲哚胺2,3-二氧化酶(IDO)的小鼠肝细胞在同种异体脾内的表达及对脾淋巴细胞的凋亡作用。方法分离BABL/c小鼠肝细胞,肝细胞脾内移植(BABL/c→C57BL/6),分为4组:Ⅰ组为携带IDO组;Ⅱ组为1-甲基色氨酸组;Ⅲ组为空壳腺病毒组;Ⅳ组为单纯肝细胞移植组。移植术后第2,5d取脾脏免疫组织化学检测IDO表达情况,TUNEL检测脾脏淋巴细胞凋亡情况。结果携带IDO腺病毒转染肝细胞后,能有效表达IDO。免疫组织化学显示,Ⅰ、Ⅱ组脾脏有IDO阳性的肝细胞。与其他组比较,移植术后第2,5dⅠ组淋巴细胞凋亡差别有统计学意义(P<0.05),Ⅱ、Ⅲ、Ⅳ组之间比较,差别无统计学意义(P>0.05)。结论携带IDO的肝细胞能在小鼠同种异体脾内稳定表达,并能诱导脾内淋巴细胞的凋亡。Objective To evaluate the effects of Indoleamine 2 ,3-dioxygenase （IDO） on the mice allogeneic hepatocytes transplanted into spleen . Methods BABL/c mice hepatocytes were isolated and transplanted into C57BL/6 mouse and divided into IDO group （I group ） , IDO ＋ 1-methyl tryptophan （1-MT） group（Ⅱ group） ,shell adenovirus group（Ⅲ group） and hepatocyte group（Ⅳ group） . The IDO was detected by immunohistochemistry and lymphocyte apoptosis of spleen were detected by TUNEL on the day 2 ,5 after transplantation . Results Hepatocytes effectively expressed IDO after transfection . Immunohistochemistry showed that IDO-positive hepatocytes were observed in Ⅰ and Ⅱ group . Com-pared with other groups ,the difference of lymphocyte apoptosis in Ⅰ group was statistically significant on the day 2 ,5 after transplantation（P＆lt;0 .05） ,no difference were observed between Ⅱ ,Ⅲ and Ⅳ groups （P＆gt;0 .05） . Conclusion Mice allogeneic hepatocytes transfected with IDO can stably express IDO and induce lymphocyte apoptosis in spleen .

关 键 词：色氨酸加氧酶 肝细胞 淋巴细胞 细胞凋亡 细胞移植 脾 疾病模型  动物 

分 类 号：R617[医药卫生—外科学] R332[医药卫生—临床医学]