局部湿热对小鼠乳腺癌组织中miRNA-7和miRNA-126表达的影响  

Effects of Local Wet Hyperthermia on the Expression of miRNA-7 and miRNA-126 in Mouse Mammary Carcinoma

作　　者：周涯[1] 李永菊[2] 周洪练[1] 任社华[1] 

机构地区：[1]遵义医学院医学物理学教研室,贵州遵义563000 [2]遵义医学院免疫学教研室,贵州遵义563000

出　　处：《黔南民族医专学报》2013年第4期235-238,243,共5页Journal of Qiannan Medical College for Nationalities

基　　金：贵州省卫生厅项目(D-348);遵义市红花岗区科技项目(E-110)

摘　　要：目的:观察局部湿热对乳腺癌荷瘤小鼠肿瘤局部miRNA-7和miRNA-126表达的影响,并探讨其意义。方法:建立小鼠乳腺癌实验动物模型,进行局部湿热处理。28 d后,用Real-time PCR探针法分别检测肿瘤局部miRNA-7和miRNA-126的表达变化;同时用TUNEL法检测肿瘤细胞凋亡变化。结果:与对照组相比,局部湿热处理组中肿瘤组织miRNA-7和miRNA-126的表达均显著上调(P<0.05)。同时,肿瘤局部细胞凋亡率也明显增加(P<0.05)。结论:湿热治疗可改变肿瘤局部中特定miRNAs分子的表达,这可能是其导致肿瘤细胞凋亡的重要原因。Objective： To observe the effects of local wet hyperthermia on the expression of miRNA -7 and miRNA -126 in mouse mammary carcinoma and explore its significance. Methods： An experimental ani- mal model of mouse mammary carcinoma was established and then treated with local wet hyperthermia. On day 28, the relative expression of miRNA -7 and miRNA -126 was determined by real- time PCR assay. Moreo- ver, the apoptosis of cells in tumor mass was detected by TUNEL assay. Results： The expression of miRNA - 7 and miRNA - 126 in local wet hyperthermia treated group increased significantly compared with that in the control group （P 〈 0. 05）. Moreover, the apoptosis rate of local tumor cells also increased significantly （P 〈 0. 05）. Conclusion： Wet hyperthermia treatment could alter the expression of specific miRNAs molecules, which may be closely related to enhanced apoptosis of tumor cells.

关 键 词：乳腺癌 局部湿热 凋亡 

分 类 号：R392.11[医药卫生—免疫学]