禽副黏病毒4型荧光RT-PCR检测方法的建立  

Establishment of Real-time RT-PCR Assayfor Detection of Avian Paramyxovirus Serotype 4

作　　者：王静静[1] 刘华雷[1] 王英丽[1] 郑东霞[1] 赵云玲[1] 吕艳[1] 左媛媛[1] 于松梅[1] 王志亮[1] 

机构地区：[1]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国动物检疫》2014年第3期68-71,共4页China Animal Health Inspection

基　　金：公益性行业(农业)科研专项(201303033)

摘　　要：本研究根据GenBank发表的禽副黏病毒4型(APMV-4)基因组序列,在保守区域分别设计了特异性RT-PCR引物和荧光探针,初步建立了可用于检测APMV-4的荧光RT-PCR方法。特异性试验表明本方法特异性强,与新城疫病毒(NDV)、禽流感病毒(AIV)、传染性支气管炎病毒(IBV)、减蛋综合征病毒(EDSV)等没有交叉反应。灵敏度试验表明本方法的检测下限为1 EID50,比常规RT-PCR的敏感度高100倍左右。本研究所建立的荧光RT-PCR方法具有快速、准确、灵敏等优点,可用于APMV-4样品的检测。A real-time RT-PCR assay for detection of avian paramyxovirus serotype 4 （APMV-4） was established in this study. The specific primers and probe used in the study were designed based on the conserved region ofAPMV-4 ge- nome. The specificity of real-time RT-PCR was demonstrated by detecting five different viruses including Newcastle dis- ease virus （NDV）, avian influelaza virus （AIV）, infectious bronchitis virus （IBV）, egg drop syndrome virus （EDSV） and APMV-4. The minimal detection threshold was 1 EID50, 100 times higher than conventional RT-PCR. These results indicated that the real-time RT-PCR would be an effective assay for the rapid detection of APMV-4.

关 键 词：禽副粘病毒4型 荧光RT-PCR 特异性 灵敏度 

分 类 号：S852.695.5[农业科学—基础兽医学]