海南养殖方斑东风螺暴发性疾病病原分离鉴定及药敏分析  

Isolation,identification and antibiotic sensitivity analysis of bacterial pathogen from cultured Babylonia areolata with fulminant infectious disease in Hainan

作　　者：刘巧红 王世锋 蔡岩 张晗 谢珍玉 郭伟良 冯永勤 周永灿 

机构地区：[1]热带生物资源可持续利用省部共建国家重点实验室培育基地海南省热带水生生物技术重点实验室,海口570228

出　　处：《渔业科学进展》2014年第1期74-81,共8页Progress in Fishery Sciences

基　　金：国家海洋公益性项目(201205025);海南省重点科技计划项目(ZDXM20110010);海南省自然科学基金(312069)共同资助

摘　　要：2011年4月,从海南工厂化养殖的患病方斑东风螺体内分离得到3株优势菌,经感染实验确定菌株DFL11-01为该暴发性疾病的致病菌,其对方斑东风螺注射感染的LD50为2.6×106CFU/g。采用常规形态和生理生化特征,对菌株DFL11-01进行鉴定,并以16SrRNA基因序列同源性分析法对所有3株分离菌进行同源性分析,结果表明,3株细菌均为哈氏弧菌Vibrio harveyi。药敏试验结果表明,菌株DFL11-01对阿洛西林、利福平等15种药物耐药;对头孢他啶、四环素等3种药物中介敏感;对氨苄西林、恩诺沙星、头孢三嗪、哌拉西林、左旋氧氟沙星等5种药物敏感。Three strains of pathogenic bacteria, DFIA1-01, DFL11-02 and DFL11-03 were isolated from the diseased Babylonia areolata cultured in Hainan Province, and were identified as Vibrio harweyi by regular physiological and biochemical observations and 16S rRNA gene se quence phylogenetic analysis. The DFL11-01 was identified as the causative agent via artificial infection test, and the LDs0 value was 2.6 X l0^6 CFU/g. Drug sensitivny tests showed that strain DFLll-01 was highly sensitive to ampicillin, ceftriaxone, piperacillin, enrofloxacin and lebofloxacin; mildly sensitive to neomycin, tetracycline, and ce{tazidime, but resistant to 15 kinds of antibiotics including azlocillin,rifampir and cefaclor.

关 键 词：方斑东风螺 哈氏弧菌 分离鉴定 致病性 药敏分析 

分 类 号：S944.49[农业科学—水产养殖]