5-HT_(1B/1D)受体亚型在大鼠三叉神经节及肺动脉表达差异性研究  

Study on the Differential Expression of 5-HT_(1B/1D) Receptor Genes in Rat Trigeminal Ganglion and Pulmonary Artery

作　　者：董旭[1] 王怀良[1] 章新华[1] 邢军[1] 魏金荣[2] 

机构地区：[1]中国医科大学基础医学院临床药理学教研室,沈阳110001 [2]中国医科大学生物技术研究所

出　　处：《中国医科大学学报》2001年第1期6-8,共3页Journal of China Medical University

基　　金：科技部重点资助项目 !96-90 1-0 5 -2 74;卫生部科研基金资助项目 !98-1-179

摘　　要：目的 :研究大鼠三叉神经节与肺动脉 5 - TH1 B/1 D受体亚型基因表达 ,探讨肺动脉高压的 5 - HT受体机制。方法 :应用 RT- PCR技术研究 5 - HT1 B和 5 - HT1 D受体基因在大鼠三叉神经节及肺动脉的表达。结果 :5 - HT1 B和 5 -HT1 D受体 m RNA在大鼠三叉神经节均有表达 ,而在大鼠肺动脉只检测到 5 - HT1 B受体 m RNA,且其表达水平在慢性肺动脉高压大鼠肺动脉明显高于正常大鼠肺动脉。结论 :5 - HT1 B和 5 - HT1 D受体在大鼠三叉神经节和肺动脉的分布存在差异 ;慢性肺动脉高压大鼠肺动脉 5 - HT1 B受体 mObjective: Through detecting the expression of 5 HT 1B/1D receptor subtype mRNAs in rat trigeminal ganglion (TG) and pulmonary artery (PA) in order to understand the 5 HT receptor mechanism of pulmonary hypertension. Methods: RT PCR technique was performed to identify the expression of 5 HT 1B and 5 HT 1D receptor mRNAs in rat TG and PA. Results: Both 5 HT 1B and 5 HT 1D receptor mRNAs were detected in rat TG, while only 5 HT 1B receptor mRNA in rat PA. A significantly higher level of 5 HT 1B receptor mRNA was detected in PA from rat with chronic pulmonary hypertension. Conclusion: The distributions of 5 HT 1B and 5 HT 1D receptors were of difference in rat TG and PA. The higher level of expression of 5 HT 1B receptor was detected in PA of chronic pulmonary hypertensive rat.

关 键 词：5-HT1B/1D受体 逆转录聚合酶链反应 三叉神经节 肺动脉高压 

分 类 号：R543.3[医药卫生—心血管疾病] R971[医药卫生—内科学]