广西斑茅遗传多样性的SCoT标记分析  

Genetic Diversity Analysis of Saccharum arundinaceum Revealed by SCoT Markers

作　　者：宋焕忠[1,2] 张荣华 杨海霞[1,2] 张革民 罗霆 高轶静[1,2] 刘昔辉 段维兴[1,2] 韦霖斌 方位宽[1,2] 危晓丽 黄玉新[1,2] 

机构地区：[1]广西农业科学院甘蔗研究所,广西南宁530007 [2]国家糖料改良中心广西甘蔗品种改良分中心,广西南宁530007 [3]昭平县市容市政局园林所,广西贺州546800

出　　处：《西南农业学报》2014年第1期59-64,共6页Southwest China Journal of Agricultural Sciences

基　　金：广西自然科学基金项目(2010GXNSFD013034;2010GXNSFB013027;2011GXNSFF018002;2012GXNSFBA0530 36);广西科学研究与技术开发计划项目(桂科能1131B01);广西农业科学院科技发展基金项目(桂农科2011JM06);广西农科院基本科研业务专项项目(桂农科2011YT01;桂农科2011YT04;桂农科2012YZ16;桂农科2012YM12;桂农科2012YM14);广西甘蔗研究所基本科研业务专项项目(G2009010)

摘　　要：利用SCoT分子标记技术对采自广西的50份斑茅无性系进行遗传多样性分析,从46条引物中筛选出扩增结果稳定、条带清晰和有多态性的引物15条,扩增位点336个,其中多态性位点284个,多态性为83.93%,说明广西斑茅具有较丰富的遗传多样性。根据研究结果进行聚类分析,在相似系数0.73的水平,可将50份斑茅无性系归为4大类;在相似系数0.76的水平,可将第Ⅳ类分成6种类型,同一地区的斑茅无性系基本聚在同一类,呈现出一定的地域性分布规律。In this paper,we conducted genetic analysis of 50 Saccharum arundinaceum germplasms by using SCoT markers.The results showed that 15 bands with 336 locus including 284 polymorphic sites were amplified by using 16 pairs of polymorphic primers.Polymorphism was 83.93 ％ which indicated that S.arundinaceum gennplasma had abundant diversity in Guangxi.The UPGMA clustering analysis showed that 50 S.arundinaceum could be divided into four major groups on similarity coeffcient level of 0.73,and germplssma could be divided into six major groups on similarity coefficient level of 0.76.S.arundinaceum germplasms from the same region were basically clustered together,which presented regional distribution to some extent.

关 键 词：斑茅 SCoT标记 遗传多样性 广西 

分 类 号：S566.1[农业科学—作物学]