红河州烟草苗期烟草花叶病毒的检测与分析  

Detection and Analysis of TMV in Floating System of Tobacco in Honghe Tobacco Area

作　　者：陈永对[1] 李宏光[2] 刘春明[2] 董家红[1] 肖俊华[3] 张仲凯[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室,云南昆明650223 [2]云南省烟草公司红河州公司技术中心,云南弥勒652300 [3]云南省烟草公司红河州公司泸西分公司,云南泸西652400

出　　处：《西南农业学报》2014年第1期156-159,共4页Southwest China Journal of Agricultural Sciences

基　　金：云南省烟草公司红河州公司项目(HYSWZXY2011-19);云南省烟草公司科技计划项目(HYSWZ2013-0134)

摘　　要：及时剔除带毒烟苗是防控烟草花叶病的重要一环,该研究采用TAS-ELISA检测与电镜负染色法复检相结合,对2011年红河烟区1308份苗期样品进行TMV检测。结果表明,红河烟区不同育苗点烟苗TMV检出率差异较大,平均检出率为2.52%;不同烤烟品种烟苗带毒率也有差异,烟苗TMV阳性率与品种的抗性无直接相关性,而与育苗地点和育苗环境有关。该研究对筛选无毒壮苗,切断TMV传播途径、减少TMV对大田烟苗的感染具有重要意义。Eliminating the TMV-infected seedlings is one of the moet important steps of prevention and control of tobacco mosaic virus dis-ease.In this study,1308 tobacco leaf samples at seedling stage were tested for TMV by TAS-ELISA and negative staining approach.The re-sults showed that the detection rate of TMV on seedlings was different with an average detection rate of 2.52 ％ in different nursery place.The infection rate of TMV in different flue-cured tobacco species was different,which had no cordation with species,but had correlationwith nursery place and nursery environment.The results are of great significance for screening non-infected seedlings,blocking the infectionsource,and reducing the infection of TMV on tobacco during field growing periods.

关 键 词：红河烟区 烟苗 烟草花叶病毒 检测 

分 类 号：S572[农业科学—烟草工业]