补肾和补肾健脾方对尾部悬吊大鼠股骨骨丢失的作用研究  

Effects of tonifying kidney prescription, tonifying kidney and invigorating spleen prescription on bone loss of femur in tail suspension model rats

作　　者：张林[1] 谢鑫[1] 王俊岩[1] 陈文娜[1] 刘鹏[1] 张秋华[1] 李环宇[1] 

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中华中医药杂志》2014年第3期700-703,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81001493);高等学校博士学科点专项科研基金资助课题(No.20102133120001);辽宁省教育厅基金(No.L2011162)~~

摘　　要：目的:观察补肾和补肾健脾方对尾部悬吊大鼠股骨的影响,比较它们防护骨丢失作用机制的异同。方法:采用头低位-30°尾部悬吊模拟失重性骨丢失,实验结束后,ELISA法测血清碱性磷酸酶(ALP)活性、胰岛素样生长因子(IGF-1)和骨桥蛋白(OPN)含量,计算右侧股骨矿化沉积速率,观察左侧股骨骨形态计量学静态参数。结果:与正常组比较,悬吊组大鼠血清ALP活性、IGF-1和OPN含量、矿化沉积速率、骨小梁面积比、骨小梁厚度和骨小梁数量均显著降低(P<0.01),骨小梁分离度明显增高(P<0.01);与悬吊组比较,补肾组血清ALP活性、血清IGF-1含量、矿化沉积速率、骨小梁面积比、骨小梁厚度和骨小梁数量升高(P<0.01,P<0.05),骨小梁分离度明显降低(P<0.01);补肾健脾组大鼠血清ALP活性、IGF-1含量、OPN含量、矿化沉积速率、骨小梁面积比、骨小梁厚度和骨小梁数量均显著升高(P<0.01,P<0.05),骨小梁分离度明显降低(P<0.01);较之补肾组,补肾健脾组大鼠血清ALP活性和矿化沉积速率升高(P<0.05),骨小梁分离度降低(P<0.05)。结论:补肾和补肾健脾方均对模拟失重引起的股骨骨丢失具有防护作用,以补肾健脾方作用为优,其作用机制可能与提高成骨细胞活性、促进骨形成有关。Objective： To observe the action of tonifying kidney prescription and tonifying kidney and invigorating spleen prescription on the femur, and to look for the protective mechanism in tail suspension model rats. Methods： Rats were suspended by head down tilt -30° to simulate agravity. After the experiment, the activity of ALP and the contents of IGF-1 and OPN in serum were measured respectively by ELISA in rats. The mineral apposition rate of right femur in rats was calculated. Meanwhile. bone histomorphometry static parameters of left femur in rats were measured. Results： Compared with the control group, ALP activity, contents of IGF-1 and OPN in serum, mineral apposition rate, trabecular area percentage, trabecula thickness and number decreased （P〈0.01）, and trabecular separation increased obviously in suspension group （P〈0.01）. Compared with the suspension group, ALP activity, the contents of IGF-1 in serum, mineral apposition rate, trabecular area percentage, trabecula thickness and number raised （P〈0.01, P〈0.05）, but the trabecular separation reduced significantly in tonifying kidney group （P〈0.01）. The ALP activity, the contents of IGF-1 and OPN in serum, the mineral apposition rate, trabecular area percentage, trabecula thickness and number increased （P〈0.01, P〈0.05）, and trabecular separation reduced obviously in tonifying kidney and invigorating spleen group （P〈0.01）. Compared with the tonifying kidney group, the ALP activity and the mineral apposition rate raised in tonifying kidney and invigorating spleen group （P〈0.05）, and the trabecular separation reduced （P〈0.05）. Conclusion： Both tonifying kidney prescription and tonifying kidney and invigorating spleen prescription have protective effects on the bone loss of femur induced by simulated agravity and the role of tonifying kidney and invigorating spleen prescription is superior. The effective mechanism might be possible related to improving osteoblast activity to promote bone formation.

关 键 词：补肾健脾 模拟失重 尾部悬吊 骨丢失 

分 类 号：R285.5[医药卫生—中药学]