薯蓣皂苷抑制脂多糖刺激的大鼠系膜细胞增殖及对Wnt通路的影响  

Dioscin ameliorates lipopolysaccharide-stimulated rat mesangial cell proliferation through suppressing Wnt pathway and TGF-β1 expression

作　　者：孙雪艳[1,2] 杨丽平[1] 占永立[1] 刘慧洁 文玉敏[2] 董晞[4] 冒慧敏[2] 李平[4] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]北京中医药大学,北京100029 [3]北京市顺义区中医医院,北京101300 [4]中日友好医院,北京100029

出　　处：《中华中医药杂志》2014年第3期871-874,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81102588;No.81072971)~~

摘　　要：目的:探讨薯蓣皂苷干预脂多糖刺激的大鼠系膜细胞(MC)增殖及Wnt通路的作用机制。方法:体外培养大鼠MC(HBZY-1),设对照组、脂多糖(LPS)刺激组、LPS+薯蓣皂苷(安全浓度通过检测LDH释放率得到:0.1、0.5、1.0μg/mL)组。药物干预MC 48h后,采用MTT法检测各组MC的增殖情况,采用RT-PCR法及Western blot法检测Wnt4、β-catenin、转化生长因子β1(TGF-β1)基因和蛋白在各组MC中的表达情况。结果:薯蓣皂苷可以抑制LPS所致的MC增殖,下调Wnt4、β-catinin和TGF-β1 mRNA和蛋白水平的表达(P<0.01,P<0.05),并且在安全浓度范围内其抑制作用与剂量呈正相关。结论:薯蓣皂苷可能通过抑制Wnt信号通路,下调TGF-β1表达发挥抑制大鼠MC增殖的作用。Objective： To investigate the effects and mechanism of Dioscin on lipopolysaccharide （LPS） stimulated rat mesangial ceils （MC）. Methods： MC were divided into control group, LPS stimulated group, LPS＋Dioscin （0.1, 0.5, 1.0μg/mL） group, and cultured for 48 hours. MTT assay was used to detect the proliferation of MC. RT-PCR and Western blot methods were applied to detect Wnt4, β-catenin and TGF-β1 expression of the MC. Results： Dioscin could inhibit LPS-induced MC proliferation and the expression levels of Wnt4, β-catinin and TGF-β1 （P〈0.01, P〈0.05）, in a dose dependent manner. Conclusion： Dioscin can inhibit MC proliferation, and its mechanism may be related to inhibition of the Wnt signaling pathway and reduction of TGF-β1 expression.

关 键 词：薯蓣皂苷 系膜细胞增殖 脂多糖 WNT信号通路 转化生长因子β1 

分 类 号：R692.31[医药卫生—泌尿科学]